光照强度和光周期对微藻 Nannochloropsis sp. 生长和脂质含量的影响
The influence of light intensity and photoperiod on the growth and lipid content of microalgae Nannochloropsis sp.
机构信息
Universiti Kuala Lumpur, Malaysian Institute of Chemical and Bioengineering Technology, 78000 Alor Gajah, Melaka, Malaysia.
出版信息
Bioresour Technol. 2013 Feb;129:7-11. doi: 10.1016/j.biortech.2012.11.032. Epub 2012 Nov 16.
Illumination factors such as length of photoperiod and intensity can affect growth of microalgae and lipid content. In order to optimize microalgal growth in mass culture system and lipid content, the effects of light intensity and photoperiod cycle on the growth of the marine microalgae, Nannochloropsis sp. were studied in batch culture. Nannochloropsis sp. was grown aseptically for 9 days at three different light intensities (50, 100 and 200 μmol m(-2) s(-1)) and three different photoperiod cycles (24:0, 18:06 and 12:12 h light:dark) at 23 °C cultivation temperature. Under the light intensity of 100 μmol m(-2) s(-1) and photoperiod of 18 h light: 6 h dark cycle, Nannochloropsis sp. was found to grow favorably with a maximum cell concentration of 6.5×10(7) cells mL(-1), which corresponds to the growth rate of 0.339 d(-1) after 8 day cultivation and the lipid content was found to be 31.3%.
光照因素，如光周期的长短和光照强度，会影响微藻的生长和脂质含量。为了优化大规模培养系统中微藻的生长和脂质含量，本研究采用批次培养的方式，研究了光照强度和光周期循环对海洋微藻——盐藻生长的影响。在 23°C 的培养温度下，无菌培养 9 天，分别使用三种不同的光照强度（50、100 和 200 μmol m(-2) s(-1))和三种不同的光周期循环（24:0、18:06 和 12:12 h 光照:黑暗）。结果表明，在光照强度为 100 μmol m(-2) s(-1)、光照时间为 18 h:黑暗时间为 6 h 的条件下，盐藻生长良好，最大细胞浓度为 6.5×10(7)个细胞 mL(-1)，培养 8 天后的增长率为 0.339 d(-1)，脂质含量为 31.3%。
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