Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat-Yai 90112, Thailand.
Bioresour Technol. 2012 Apr;110:510-6. doi: 10.1016/j.biortech.2012.01.125. Epub 2012 Feb 8.
Microalgae capable of accumulating high lipid content were screened in photoautotrophic, heterotrophic and mixotrophic cultures. The biomass and lipid production of all tested strains in mixotrophic culture were notably enhanced in comparison with photoautotrophic and heterotrophic cultures. Among the tested strains, marine Chlorella sp. and Nannochloropsis sp. were ideal candidates for biodiesel production because of their high lipid production. The influence of light intensity and initial glucose concentration on growth and lipid content of both strains were investigated. Although increasing light intensity and initial glucose concentration enhanced the growth of both strains, it reduced their lipid content. To produce a high amount of biomass with high lipid content, a fed-batch cultivation with stepwise increasing light intensity was performed. The lipid production by this strategy was approximately twice that of conventional batch cultivation. The main fatty acid compositions of the two microalgae were C16-C18 (>80%) which are appropriate for biodiesel production.
在光自养、异养和混合培养中筛选出能够积累高脂质含量的微藻。与光自养和异养培养相比，所有测试菌株在混合培养中的生物量和脂质产量都显著提高。在测试的菌株中，海洋小球藻和等鞭金藻由于其高脂质产量，是生物柴油生产的理想候选菌株。研究了光强和初始葡萄糖浓度对两种菌株生长和脂质含量的影响。虽然增加光强和初始葡萄糖浓度可以促进两种菌株的生长，但会降低它们的脂质含量。为了在高生物量的同时获得高脂质含量，采用逐步增加光强的分批补料培养法进行培养。这种策略的脂质产量约为传统分批培养的两倍。两种微藻的主要脂肪酸组成均为 C16-C18（＞80%），适合生物柴油生产。
