J Cataract Refract Surg. 2013 Feb;39(2):258-67. doi: 10.1016/j.jcrs.2012.09.022. Epub 2012 Dec 8.
To determine the effectiveness of femtosecond ultraviolet (UV) pulses in ablating corneal stroma in a rabbit model and to compare the healing response between eyes treated with femtosecond UV pulses and eyes treated with standard excimer photorefractive keratectomy.
Laser Research Center, Vilnius University, Vilnius, Lithuania.
Experimental study.
Myopic photoablation using a femtosecond UV solid-state laser system was applied to corneas of pigmented rabbits. Experiments in 16 eyes were performed for optimization of the laser system parameters (fluence, spot size, pulse repetition rate) and calibration of ablation rate. In 7 rabbits, deep femtosecond UV ablation (∼130 μm) in 1 eye and shallow ablation (∼30 μm) in the contralateral eye were performed. Nine rabbits received an approximately 30 μm ablation with femtosecond UV pulses in 1 eye and with a conventional excimer system in the contralateral eye. Two eyes were used as controls. The ablation process and surface-temperature dynamics were monitored and recorded. Surface quality and haze development were evaluated. Rabbits were humanely killed 0 to 6 months after surgery, and eyes were enucleated for histological examination.
Rabbit corneas ablated with femtosecond UV pulses or excimer laser radiation were similar in terms of the corneal wound-healing process, surface quality, and histology.
The experiments indicate the feasibility of clinical application of femtosecond UV lasers for stromal ablation. The ability to switch between laser harmonics allows fast changeover from infrared to the UV mode, implying that a wide range of ophthalmic procedures can be performed using a single solid-state laser device.
Ms. Gabryte and Mr. Danielius are paid employees of Light Conversion Ltd. Mr. Danielius is a shareholder of Light Conversion Ltd. No other author has a financial or proprietary interest in any material or method mentioned.
在兔模型中确定飞秒紫外（UV）脉冲消融角膜基质的有效性，并比较用飞秒 UV 脉冲治疗的眼睛与用标准准分子光屈光性角膜切削术治疗的眼睛的愈合反应。
立陶宛维尔纽斯大学激光研究中心。
实验研究。
使用飞秒 UV 固态激光系统对色素兔的角膜进行近视光消融。对 16 只眼睛进行了实验，以优化激光系统参数（能量密度、光斑大小、脉冲重复率）并校准消融速率。在 7 只兔子中，在 1 只眼中进行深度飞秒 UV 消融（约 130μm），在对侧眼中进行浅层消融（约 30μm）。9 只兔子的 1 只眼中用飞秒 UV 脉冲进行了约 30μm 的消融，对侧眼中用常规准分子系统进行了消融。2 只眼睛作为对照。监测并记录消融过程和表面温度动态。评估表面质量和雾度发展。手术后 0 至 6 个月对兔子进行安乐死，眼球被取出进行组织学检查。
用飞秒 UV 脉冲或准分子激光辐射消融的兔角膜在角膜伤口愈合过程、表面质量和组织学方面相似。
实验表明临床应用飞秒 UV 激光进行基质消融是可行的。能够在激光谐波之间切换，允许快速从红外切换到 UV 模式，这意味着可以使用单个固态激光设备进行广泛的眼科手术。
