Thyberg P, Rigler R, Svartengren K, Wiman L G
Department of Medical Biophysics, Karolinska Institute, Huddinge University Hospital, Stockholm, Sweden.
Eur Biophys J. 1990;18(2):85-91. doi: 10.1007/BF00183267.
A model of a freely rotating extended scatterer is proposed to describe light scattering from beating cilia. Gaussian rotation frequency distributions, characterized by a mean angular frequency and a standard deviation, are introduced in order to simulate intensity autocorrelation functions and to fit the model to experimental data. Thus the ciliary beats are characterized by a mean beat frequency and a standard deviation of the beat frequency distribution. The standard deviation influences the damping of the intensity autocorrelation function of light scattered from cilia. The calculated intensity autocorrelation function shows a more prominent oscillating behaviour the smaller the standard deviation of the beat frequency. The validity of the model is supported by experimental data in two ways: 1) The model fits very well to experimental data in computer evaluations, 2) Neither the model nor information obtained from measurements are dependent on the measuring angle.
提出了一个自由旋转的扩展散射体模型来描述来自跳动纤毛的光散射。引入了以平均角频率和标准差为特征的高斯旋转频率分布，以便模拟强度自相关函数并使模型与实验数据拟合。因此，纤毛跳动的特征在于平均跳动频率和跳动频率分布的标准差。标准差影响从纤毛散射的光的强度自相关函数的衰减。计算得到的强度自相关函数显示，跳动频率的标准差越小，振荡行为越明显。该模型的有效性通过实验数据以两种方式得到支持：1）在计算机评估中，该模型与实验数据拟合得非常好；2）该模型和从测量中获得的信息均不依赖于测量角度。
