Jacobsen K, Tepper J, Osmond D G
Department of Anatomy, McGill University, Montreal, Quebec, Canada.
Exp Hematol. 1990 May;18(4):304-10.
The localization of early B-lymphocyte precursor cells in the bone marrow of young mice has been studied during recovery from sublethal whole body gamma-irradiation (150 rad). Initial studies by double immunofluorescence labeling of the B-lineage-associated cell surface glycoprotein, B220, and of mu heavy chains in bone marrow cell suspensions, demonstrated a sequential wave of regeneration of early B precursor cells, pre-B cells, and B cells. Early B precursor cells expressing B220 but not mu chains were enriched at 1-3 days following irradiation. After in vivo administration of 125I-labeled monoclonal antibody 14.8 to detect B220+ cells in situ, light and electron microscope radioautography of femoral bone marrow sections revealed concentrations of labeled B220+ cells located peripherally near the cortical bone at 1-3 days following irradiation, increasing in numbers in more central areas by 5-7 days. Proliferative B220+ precursor cells were found within layers of bone-lining cells and in a subosteal area characterized by a prominent electron-dense extracellular matrix, often associated with stromal reticular cells. The results demonstrate that the precursor cells that are active in the bone marrow early in the recovery of B lymphopoiesis after gamma-irradiation are located both within and near the endosteum of the surrounding bone. The distinctive extracellular matrix and stromal cell associations noted in this region may contribute to a supportive local microenvironment for early hemopoietic progenitor cells.
在亚致死剂量全身γ射线照射（150拉德）后的恢复过程中，对幼鼠骨髓中早期B淋巴细胞前体细胞的定位进行了研究。通过对骨髓细胞悬液中B谱系相关细胞表面糖蛋白B220和μ重链进行双重免疫荧光标记的初步研究表明，早期B前体细胞、前B细胞和B细胞呈现出再生的连续波。在照射后1 - 3天，表达B220但不表达μ链的早期B前体细胞富集。在体内给予125I标记的单克隆抗体14.8以原位检测B220 + 细胞后，股骨骨髓切片的光镜和电镜放射自显影显示，照射后1 - 3天，标记的B220 + 细胞集中在靠近皮质骨的外周，到5 - 7天时在更中心区域数量增加。增殖性B220 + 前体细胞存在于骨衬细胞层内以及以突出的电子致密细胞外基质为特征的骨膜下区域，该区域常与基质网状细胞相关。结果表明，γ射线照射后B淋巴细胞生成恢复早期在骨髓中活跃的前体细胞位于周围骨的骨内膜内及其附近。在该区域观察到的独特细胞外基质和基质细胞关联可能有助于为早期造血祖细胞提供支持性的局部微环境。
