一株1,2-二氯乙烷降解菌的分离及降解特性

[Isolation, degradation characteristics of a 1,2-dichloroethane-degrading strain].

作者信息

Wang Xiao-Chun, Chen Dong-Zhi, Jin Xiao-Jun, Chen Jian-Meng

机构信息

College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China.

出版信息

Huan Jing Ke Xue. 2012 Oct;33(10):3620-6.

PMID:23233997

Abstract

A 1,2-dichloroethane-degrading bacterium T-2, which was able to utilize 1,2-dichloroethane as the sole carbon and energy source, was isolated from the activated sludge of a chemical plant in Zhejiang. According to the morphological, physiological and biochemical characteristics and sequence analysis of 16S rRNA, the strain was identified as Starkeya sp. T-2. The optimal temperature and pH for 1,2-DCA degradation were 30 degrees C and 7.0-8.0, respectively. The 1,2-DCA degrading process followed the Haldane kinetic model. The maximum specific growth rate and specific degradation rate were 0.065 h(-1) and 0.54 h(-1), respectively. The maximum tolerable concentration of 1,2-DCA for the strain was 500 mg x L(-1), and the cell yield coefficient was 0. 191 mg x mg(-1). Strain T-2 could mineralize 1,2-DCA into CO2 and H2O, with a mineralization ratio of 45%.

摘要

从浙江某化工厂的活性污泥中分离出一株能以1,2 - 二氯乙烷作为唯一碳源和能源的1,2 - 二氯乙烷降解菌T - 2。根据其形态、生理生化特征及16S rRNA序列分析，该菌株被鉴定为斯达克氏菌属（Starkeya sp.）T - 2。1,2 - 二氯乙酸（1,2 - DCA）降解的最适温度和pH分别为30℃和7.0 - 8.0。1,2 - DCA的降解过程符合哈代动力学模型。最大比生长速率和比降解速率分别为0.065 h⁻¹和0.54 h⁻¹。该菌株对1,2 - DCA的最大耐受浓度为500 mg·L⁻¹，细胞产率系数为0.191 mg·mg⁻¹。菌株T - 2能将1,2 - DCA矿化为CO₂和H₂O，矿化率为45%。