Bai Yao-hui, Zhao Cui, Xiao Ya-na, Wen Dong-hui, Tang Xiao-yan
College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China.
Huan Jing Ke Xue. 2008 Dec;29(12):3546-53.
A bacterial strain BW003, which could utilize quinoline as sole carbon, nitrogen and energy source, was isolated from the activated sludge in a coking wastewater treatment plant. According to the 16S rRNA gene sequence analysis, the strain was identified as Pseudomonas sp. Biodegradation experiments showed that the strain could degrade 192-911 mg/L of quinoline efficiently within 3-8 h, and the removal rates of quinoline were ranged from 96% to 98%. The optimum conditions for the degradation were 30 degrees C and pH 8. Metabolic products analysis revealed that at least 43% quinoline was first transformed into 2-hydroxyquinoline, 0.69% 2-hydroxyquinoline was then transformed into 2,8-dihydroxyquinoline, and subsequently into 8-hydroxycoumarin in the process of biodegradation. Additionally, at least 48% of nitrogen in quinoline was transformed into NH3-N directly and external carbon source could promote the nitrogen transformation, demonstrating that the quinoline and its metabolic products could be eliminated if controlling proper C/N ratio.
从某焦化废水处理厂的活性污泥中分离出一株细菌菌株BW003，该菌株能够以喹啉作为唯一的碳源、氮源和能源。根据16S rRNA基因序列分析，该菌株被鉴定为假单胞菌属。生物降解实验表明，该菌株能够在3-8小时内高效降解192-911mg/L的喹啉，喹啉的去除率在96%至98%之间。降解的最佳条件为30℃和pH值8。代谢产物分析表明，在生物降解过程中，至少43%的喹啉首先转化为2-羟基喹啉，0.69%的2-羟基喹啉随后转化为2,8-二羟基喹啉，随后再转化为8-羟基香豆素。此外，喹啉中至少48%的氮直接转化为NH3-N，外加碳源可促进氮的转化，表明控制适当的碳氮比可消除喹啉及其代谢产物。
