支气管败血波氏杆菌皮肤坏死毒素纯化的简化程序
Simplified procedure for purification of Bordetella bronchiseptica dermonecrotic toxin.
作者信息
Horiguchi Y, Nakai T, Kume K
机构信息
Research Center for Veterinary Science, Kitasato Institute, Chiba, Japan.
出版信息
FEMS Microbiol Lett. 1990 Jan 1;54(1-3):39-43. doi: 10.1016/0378-1097(90)90255-o.
Bordetella bronchiseptica dermonecrotic toxin was purified by a simplified method. The method consisted of SP Toyopearl 650M chromatography and high performance liquid chromatography on a TSK gel G3000SW column. 47.5% of the activity of the crude cell extract was recovered. The purified toxin behaved as a homogeneous protein in sodium dodecyl sulfate polyacrylamide gel electrophoresis, high performance liquid chromatography, and agar gel double diffusion tests.
支气管败血波氏杆菌皮肤坏死毒素通过一种简化方法进行纯化。该方法包括SP Toyopearl 650M色谱法和在TSK凝胶G3000SW柱上的高效液相色谱法。粗细胞提取物47.5%的活性得以回收。纯化后的毒素在十二烷基硫酸钠聚丙烯酰胺凝胶电泳、高效液相色谱和琼脂凝胶双向扩散试验中表现为单一蛋白质。
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