Horiguchi Y, Sugimoto N, Matsuda M
Department of Tuberculosis Research, Osaka University, Japan.
Infect Immun. 1993 Sep;61(9):3611-5. doi: 10.1128/iai.61.9.3611-3615.1993.
We investigated the effects of Bordetella bronchiseptica dermonecrotic toxin on DNA synthesis in MC3T3-E1 cells. The rate of [methyl-3H]thymidine incorporation increased in the toxin-treated cells more than 24 h after addition of the toxin under the serum-starved conditions. This effect was dependent on the toxin concentration ranging from 0.3 to 3 ng/ml and was eliminated by aphidicolin and hydroxyurea, inhibitors for DNA replication. In the toxin-treated culture, the number of cells did not increase but polynucleated cells appeared and their number increased to ca. 50% of the total number of cells 6 days after the toxin addition. From these results, we concluded that the toxin stimulates DNA replication in MC3T3-E1 cells without cell proliferation.
我们研究了支气管败血波氏杆菌皮肤坏死毒素对MC3T3-E1细胞DNA合成的影响。在血清饥饿条件下，添加毒素超过24小时后，毒素处理的细胞中[甲基-3H]胸苷掺入率增加。这种效应依赖于0.3至3 ng/ml的毒素浓度，并且被DNA复制抑制剂阿非科林和羟基脲消除。在毒素处理的培养物中，细胞数量没有增加，但出现了多核细胞，并且在添加毒素6天后，它们的数量增加到细胞总数的约50%。从这些结果中，我们得出结论，该毒素刺激MC3T3-E1细胞中的DNA复制而不引起细胞增殖。
