Department of Poultry Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000, Zagreb, Croatia.
Avian Pathol. 2012 Dec;41(6):555-62. doi: 10.1080/03079457.2012.733340.
The natural occurrence of chicken and turkey-origin astroviruses in domestic ducks (Anas platyrhynchos domesticus) is described. Twenty-two duck flocks were covered by this research. The liver, spleen, kidney and intestines were sampled and tested by reverse-transcription polymerase chain reaction for the presence of avian nephritis virus (ANV), chicken astrovirus (CAstV), turkey astrovirus (TAstV)-1, TAstV-2 and duck astrovirus. The astrovirus infection was confirmed in multiple organ samples from 59.1% of tested flocks. CAstV was detected in one flock, TAstV-2 in three flocks and ANV in 10 flocks. The molecular and phylogenetic analysis of the small open reading frame (ORF) 1b fragment (130 nucleotides) of all chicken and turkey-origin astroviruses detected in ducks showed that ANV-sequence group was more distant from CastV, TAstV-1 and TAstV-2 sequences, which formed a separate, more related group. ANV sequences were divided into three subgroups, suggesting that several types of ANV were circulating in Croatian duck flocks. The comparison of the partial ORF 1b (254 nucleotides) duck ANV sequences with 21 ANVs detected in various avian species (chickens, turkeys, geese, guinea fowl and pigeons) revealed they shared the higher nucleotide (95.6 to 97.2%) and amino acid (98.8 to 100%) identity with two ANV-2-like sequences from chickens (GA-SEP-A451-05 and GA-CK-SEP ANV-364-2005). Phylogenetic neighbour-joining tree analysis based on the same nucleotide alignment, and performed using the Jukes-Cantor method, clustered the compared sequences into three groups. All analysed duck ANV sequences showed a close phylogenetic relationship with chicken-origin ANVs. Additional work is required to determine the significance and pathogenicity of chicken and turkey-origin astroviruses in domestic ducks.
描述了鸡和火鸡源星状病毒在国内鸭（Anas platyrhynchos domesticus）中的自然发生情况。本研究涵盖了 22 个鸭群。通过逆转录聚合酶链反应检测肝脏、脾脏、肾脏和肠道中禽肾炎病毒（ANV）、鸡星状病毒（CAstV）、火鸡星状病毒（TAstV）-1、TAstV-2 和鸭星状病毒的存在情况。在 59.1%的检测鸭群的多个器官样本中证实了星状病毒感染。在一个鸭群中检测到 CAstV，在三个鸭群中检测到 TAstV-2，在 10 个鸭群中检测到 ANV。对在鸭中检测到的所有鸡和火鸡源星状病毒的小开放阅读框（ORF）1b 片段（130 个核苷酸）进行分子和系统发育分析表明，ANV 序列组与 CAstV、TAstV-1 和 TAstV-2 序列的距离更远，形成了一个单独的、更相关的组。ANV 序列分为三个亚群，表明几种类型的 ANV 在克罗地亚鸭群中循环。对部分 ORF 1b（254 个核苷酸）鸭 ANV 序列与在各种禽类（鸡、火鸡、鹅、珍珠鸡和鸽子）中检测到的 21 种 ANV 进行比较表明，它们与两种鸡源 ANV-2 样序列（GA-SEP-A451-05 和 GA-CK-SEP ANV-364-2005）共享更高的核苷酸（95.6%至 97.2%）和氨基酸（98.8%至 100%）同一性。基于相同核苷酸比对的系统发育近邻-joining 树分析，并使用 Jukes-Cantor 方法进行分析，将比较的序列聚类为三组。所有分析的鸭 ANV 序列与鸡源 ANV 具有密切的系统发育关系。需要进一步研究确定鸡和火鸡源星状病毒在国内鸭中的意义和致病性。
