Division of Environmental Science and Engineering, National University of Singapore, Singapore.
FEMS Microbiol Ecol. 2013 May;84(2):259-69. doi: 10.1111/1574-6941.12057. Epub 2013 Jan 15.
Knowledge about the biogeography of marine bacterioplankton on the global scale in general and in Southeast Asia in particular has been scarce. This study investigated the biogeography of bacterioplankton community in Singapore seawaters. Twelve stations around Singapore island were sampled on different schedules over 1 year. Using PCR-DNA fingerprinting, DNA cloning and sequencing, and microarray hybridization of the 16S rRNA genes, we observed clear spatial variations of bacterioplankton diversity within the small area of the Singapore seas. Water samples collected from the Singapore Strait (south) throughout the year were dominated by DNA sequences affiliated with Cyanobacteria and Alphaproteobacteria that were believed to be associated with the influx of water from the open seas in Southeast Asia. On the contrary, water in the relatively polluted Johor Strait (north) were dominated by Betaproteobacteria, Gammaproteobacteria, and Bacteroidetes and that were presumably associated with river discharge and the relatively eutrophic conditions of the waterway. Bacterioplankton diversity was temporally stable, except for the episodic surge of Pseudoalteromonas, associated with algal blooms. Overall, these results provide valuable insights into the diversity of bacterioplankton communities in Singapore seas and the possible influences of hydrological conditions and anthropogenic activities on the dynamics of the communities.
关于海洋细菌浮游生物在全球范围内特别是在东南亚地区的生物地理学知识一直很匮乏。本研究调查了新加坡海域细菌浮游生物群落的生物地理学。在一年的不同时间，在新加坡岛周围的 12 个站点进行了采样。通过 PCR-DNA 指纹分析、DNA 克隆和测序以及 16S rRNA 基因的微阵列杂交，我们观察到了新加坡海域小范围内细菌浮游生物多样性的明显空间变化。全年从新加坡海峡（南部）采集的水样主要由蓝细菌和α变形菌的 DNA 序列组成，这些细菌被认为与来自东南亚开阔海域的水流入有关。相反，在相对污染的柔佛海峡（北部）的水中，β变形菌、γ变形菌和拟杆菌占主导地位，这可能与河流排放和水道的相对富营养化条件有关。细菌浮游生物多样性在时间上是稳定的，除了与藻类大量繁殖有关的假交替单胞菌的偶发性激增。总的来说，这些结果为了解新加坡海域细菌浮游生物群落的多样性以及水文学条件和人为活动对群落动态的可能影响提供了有价值的见解。
