被单克隆抗体5T4识别的滋养层糖蛋白定位于人类染色体6q14 - q15。
Trophoblast glycoprotein recognised by monoclonal antibody 5T4 maps to human chromosome 6q14-q15.
作者信息
Boyle J M, Grzeschik K H, Heath P R, Morten J E, Stern P L
机构信息
Department of Biochemical Genetics, Paterson Institute of Cancer Research, Christie Hospital and Holt Radium Institute, Manchester, UK.
出版信息
Hum Genet. 1990 Apr;84(5):455-8. doi: 10.1007/BF00195819.
Human X rodent hybrids were stained by indirect immunofluorescence with 5T4, a murine monoclonal antibody that recognises a 72 kdalton glycoprotein expressed by human trophoblasts and a very restricted range of adult tissues; they were analysed by flow cytometry. Concordance analysis supported by segregation data allowed assignment of the gene controlling glycoprotein expression (M6P1) to chromosome 6. Similar analysis with translocation hybrids gave a regional assignment to 6q14-q15. M6P1 is distinct from NT5, coding for 5' nucleotidase, which maps to the same region.
用人鼠杂交细胞通过间接免疫荧光法用5T4进行染色，5T4是一种鼠单克隆抗体，可识别由人滋养层细胞和非常有限范围的成人组织表达的72kDa糖蛋白；通过流式细胞术对它们进行分析。由分离数据支持的一致性分析允许将控制糖蛋白表达的基因（M6P1）定位到6号染色体。用易位杂交细胞进行的类似分析将其区域定位到6q14 - q15。M6P1与编码5'核苷酸酶的NT5不同，NT5定位于同一区域。
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