Division of Sustainable Energy and Environmental Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Biodegradation. 2013 Sep;24(5):665-74. doi: 10.1007/s10532-012-9614-1. Epub 2012 Dec 13.
Four novel metabolic 1,4-dioxane degrading bacteria possessing high ability to degrade 1,4-dioxane (designated strains D1, D6, D11 and D17) were isolated from soil in the drainage area of a chemical factory. Strains D6, D11 and D17 were allocated to Gram-positive actinomycetes, similar to previously reported metabolic 1,4-dioxane degrading bacteria, whereas strain D1 was allocated to Gram-negative Afipia sp. The isolated strains could utilize a variety of carbon sources, including cyclic ethers, especially those with carbons at position 2 that were modified with methyl- or carbonyl-groups. The cell yields on 1,4-dioxane were relatively low (0.179-0.223 mg-protein (mg-1,4-dioxane)(-1)), which was likely due to requiring energy for C-O bond fission. The isolated strains showed 2.6-13 times higher specific 1,4-dioxane degradation rates (0.052-0.263 mg-1,4-dioxane (mg-protein)(-1) h(-1)) and 2.3-7.8 fold lower half saturation constants (20.6-69.8 mg L(-1)) than the most effective 1,4-dioxane degrading bacterium reported to date, Pseudonocardia dioxanivorans CB1190, suggesting high activity and affinity toward 1,4-dioxane degradation. Strains D1 and D6 possessed inducible 1,4-dioxane degrading enzymes, whereas strains D11 and D17 possessed constitutive ones. 1,4-Dioxane degradation (100 mg L(-1)) by Afipia sp. D1 was not affected by the co-existence of up to 3,000 mg L(-1) of ethylene glycol. The effects of initial pH, incubation temperature and NaCl concentration on 1,4-dioxane degradation by the four strains revealed that they could degrade 1,4-dioxane under a relatively wide range of conditions, suggesting that they have a certain adaptability and applicability for industrial wastewater treatment.
从化工厂排水区的土壤中分离到四株具有高效降解 1,4-二恶烷能力的新型代谢 1,4-二恶烷细菌（命名为菌株 D1、D6、D11 和 D17）。菌株 D6、D11 和 D17 被归类为革兰氏阳性放线菌，类似于先前报道的代谢 1,4-二恶烷降解细菌，而菌株 D1 则被归类为革兰氏阴性 Afipia sp.。分离出的菌株可以利用多种碳源，包括环状醚，特别是那些在位置 2 上用甲基或羰基修饰的碳源。细胞在 1,4-二恶烷上的产率相对较低（0.179-0.223 mg-蛋白（mg-1,4-二恶烷）-1），这可能是由于需要能量来断裂 C-O 键。与迄今为止报道的最有效的 1,4-二恶烷降解菌 Pseudonocardia dioxanivorans CB1190 相比，分离出的菌株表现出 2.6-13 倍更高的特定 1,4-二恶烷降解率（0.052-0.263 mg-1,4-二恶烷（mg-蛋白）-1 h-1）和 2.3-7.8 倍更低的半饱和常数（20.6-69.8 mg L-1），表明它们对 1,4-二恶烷降解具有高活性和亲和力。菌株 D1 和 D6 具有诱导性 1,4-二恶烷降解酶，而菌株 D11 和 D17 则具有组成性 1,4-二恶烷降解酶。在共存高达 3000 mg L-1 的乙二醇的情况下， Afipia sp. D1 对 100 mg L-1 1,4-二恶烷的降解不受影响。四株菌对初始 pH 值、培养温度和 NaCl 浓度对 1,4-二恶烷降解的影响表明，它们可以在相对较宽的条件下降解 1,4-二恶烷，这表明它们对工业废水处理具有一定的适应性和适用性。
