Kim Young-Mo, Jeon Jong-Rok, Murugesan Kumarasamy, Kim Eun-Ju, Chang Yoon-Seok
School of Environmental Science and Engineering, POSTECH, Pohang, South Korea.
Biodegradation. 2009 Jul;20(4):511-9. doi: 10.1007/s10532-008-9240-0. Epub 2008 Dec 17.
A new bacterial strain PH-06 was isolated using enrichment culture technique from river sediment contaminated with 1,4-dioxane, and identified as belonging to the genus Mycobacterium based on 16S rRNA sequencing (Accession No. EU239889). The isolated strain effectively utilized 1,4-dioxane as a sole carbon and energy source and was able to degrade 900 mg/l 1,4-dioxane in minimal salts medium within 15 days. The key degradation products identified were 1,4-dioxane-2-ol and ethylene glycol, produced by monooxygenation. Degradation of 1,4-dioxane and concomitant formation of metabolites were demonstrated by GC/MS analysis using deuterium labeled 1,4-dioxane (1,4-dioxane-d8). In addition to 1,4-dioxane, this bacterium could also transform structural analogues such as 1,3-dioxane, cyclohexane and tetrahydrofuran when pre-grown with 1,4-dioxane as the sole growth substrate. Our results suggest that PH-06 can maintain sustained growth on 1,4-dioxane without any other carbon sources.
采用富集培养技术，从受1,4 - 二氧六环污染的河流沉积物中分离出一种新的细菌菌株PH - 06，并基于16S rRNA测序（登录号EU239889）鉴定其属于分枝杆菌属。分离出的菌株能有效利用1,4 - 二氧六环作为唯一碳源和能源，在基本盐培养基中15天内能够降解900 mg/L的1,4 - 二氧六环。鉴定出的关键降解产物是通过单加氧作用产生的1,4 - 二氧六环 - 2 - 醇和乙二醇。使用氘标记的1,4 - 二氧六环（1,4 - 二氧六环 - d8）通过气相色谱/质谱分析证明了1,4 - 二氧六环的降解及代谢产物的同时形成。除了1,4 - 二氧六环外，当该细菌以1,4 - 二氧六环作为唯一生长底物预培养时，它还能转化结构类似物，如1,3 - 二氧六环、环己烷和四氢呋喃。我们的结果表明，PH - 06可以在没有任何其他碳源的情况下，以1,4 - 二氧六环维持持续生长。
