College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, PR China.
J Sci Food Agric. 2013 Jun;93(8):1979-85. doi: 10.1002/jsfa.6003. Epub 2012 Dec 12.
Cooking has a great influence on meat tenderness, and we assumed that changes of tenderness in this process resulted from the liberation of actin and associated structural changes. Therefore, in this study, we investigated changes in the microstructure and tenderness when duck breast muscle were cooked to different internal endpoint temperatures (70, 80, 90, 95 °C) by three cooking regimes (conventional cooking, two-stage cooking and three-stage cooking).
When duck breast muscle was cooked to an internal endpoint temperature of 70 °C, the three-stage cooking significantly decreased the Meullenet-Owens razor shear force (MORSF) value (P<0.05) and resulted in a longer sarcomere length (P<0.05) compared with the conventional cooking. However, further improvement of the tenderness and an increase in sarcomere length did not occur at higher internal endpoint temperatures. The two-stage cooking regime, with a much longer cooking time below 55 °C, also resulted in a lower MORSF value (P>0.05) and increased sarcomere length (P<0.05) at an internal endpoint temperature of 70 °C compared with the conventional cooking. Significant liberation of actin was also observed in duck meat cooked between 50 and 60 °C.
The two- and three-stage cooking regimes with initial temperatures of 50-60 °C and endpoint temperature of 70 °C could improve tenderness of duck breast meat, which might be related to the liberation of actin. Three-stage cooking had the great advantages of improving meat tenderness and reducing cooking loss; therefore, this is to be recommended for further application and research.
烹饪对肉的嫩度有很大的影响,我们假设在这个过程中嫩度的变化是由于肌动蛋白的释放和相关的结构变化引起的。因此,在这项研究中,我们通过三种烹饪方式(常规烹饪、两段式烹饪和三段式烹饪),研究了当鸭胸肉被烹饪到不同的内部终点温度(70、80、90、95°C)时,微观结构和嫩度的变化。
当鸭胸肉被烹饪到 70°C 的内部终点温度时,与常规烹饪相比,三段式烹饪显著降低了肌球蛋白-Owens 剃刀剪切力(MORSF)值(P<0.05),并导致肌节长度变长(P<0.05)。然而,在更高的内部终点温度下,嫩度的进一步改善和肌节长度的增加并没有发生。两段式烹饪,在 55°C 以下的烹饪时间更长,也导致了在 70°C 的内部终点温度下更低的 MORSF 值(P>0.05)和更长的肌节长度(P<0.05),与常规烹饪相比。在 50-60°C 之间烹饪的鸭肉中也观察到肌动蛋白的显著释放。
初始温度为 50-60°C 和终点温度为 70°C 的两段式和三段式烹饪方式可以改善鸭胸肉的嫩度,这可能与肌动蛋白的释放有关。三段式烹饪在提高肉质嫩度和减少烹饪损失方面具有很大的优势;因此,建议进一步应用和研究。