Department of Biomedical Sciences, University of Cagliari, Cittadella Universitaria di Monserrato, Cagliari, Italy.
Cell Tissue Res. 2013 Mar;351(3):409-17. doi: 10.1007/s00441-012-1533-2. Epub 2012 Dec 14.
We compared changes in the morphology of mitochondrial cristae with those in the blood and adrenal content of steroid hormones after the stimulation or inhibition of steroidogenesis. Rats were treated with adrenocorticotrophic hormone or angiotensin II to elicit steroidogenesis and with dexamethasone to inhibit it. Blood and adrenal glands were collected after several time intervals for measurements of steroids and their main intermediates. In the zona fasciculata, mitochondrial ultrastructure was investigated by high resolution scanning electron microscopy. We found that the morphometric data correlated well with the measurements of hyper- or hypo-activity of steroidogenesis over short periods of time (4 h) but not over longer observation times. A peculiar finding was that, contrary to previous reports, 11-deoxycortisol was present in adult rat adrenal tissue.
我们比较了刺激或抑制类固醇生成后，线粒体嵴形态的变化与血液和肾上腺类固醇激素含量的变化。用促肾上腺皮质激素或血管紧张素 II 处理大鼠以引发类固醇生成，用地塞米松抑制其生成。在不同的时间间隔后收集血液和肾上腺，以测量类固醇及其主要中间产物。在束状带中，通过高分辨率扫描电子显微镜研究了线粒体的超微结构。我们发现，形态计量学数据与短时间（4 小时）内类固醇生成的高或低活性的测量结果密切相关，但与较长时间的观察结果无关。一个特别的发现是，与以前的报道相反，11-脱氧皮质醇存在于成年大鼠的肾上腺组织中。
