Dimri Umesh, Sharma Mahesh Chandra, Singh Shanker K, Kumar Pankaj, Jhambh Ricky, Singh Bishwambhar, Bandhyopadhyay Samiran, Verma Med Ram
Division of Medicine, Indian Veterinary Research Institute, Izatnagar, 243122, Bareilly, UP, India.
Trop Anim Health Prod. 2013 Apr;45(4):971-8. doi: 10.1007/s11250-012-0319-6. Epub 2012 Dec 13.
The effect of vitamins A, D3, E, and H supplementation on oxidative stress indices in Indian water buffaloes suffering from subclinical mastitis was investigated. Changes in the total oxidant capacity (TOC), total antioxidant capacity (TAC), nitric oxide (NO), and activities of glutathione peroxidase (GSH-Px), catalase (CAT), and superoxide dismutase (SOD) in milk were evaluated before and after the supplementation of vitamins A, D3, E, and H. The buffaloes suffering from subclinical mastitis revealed remarkable alterations in the milk oxidants/antioxidants balance shifted towards oxidative status. The buffaloes with subclinical mastitis revealed significantly (P ≤ 0.01) higher TOC, NO contents, and CAT activity, while TAC content and GSH-Px activity were significantly (P ≤ 0.01) lower in comparison with the healthy controls. However, SOD activity did not show any significant change. Supplementation of vitamins A, D3, E, and H to these animals revealed significant (P ≤ 0.01) reduction in TOC, NO, and CAT, while a significant (P ≤ 0.01) increase in TAC and GSH-Px activity was also evident. From the present study, it may be concluded that supplementation of these vitamins can help ameliorate the altered milk oxidants/antioxidants balance towards normalcy and, thus, ensue recovery from subclinical mastitis in the Indian water buffaloes.
研究了补充维生素A、D3、E和H对患有亚临床乳腺炎的印度水牛氧化应激指标的影响。在补充维生素A、D3、E和H之前和之后，评估了牛奶中总氧化能力（TOC）、总抗氧化能力（TAC）、一氧化氮（NO）以及谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）活性的变化。患有亚临床乳腺炎的水牛乳汁中氧化剂/抗氧化剂平衡向氧化状态转变，显示出显著变化。与健康对照组相比，患有亚临床乳腺炎的水牛TOC、NO含量和CAT活性显著更高（P≤0.01），而TAC含量和GSH-Px活性显著更低（P≤0.01）。然而，SOD活性没有显示出任何显著变化。给这些动物补充维生素A、D3、E和H后，TOC、NO和CAT显著降低（P≤0.01），同时TAC和GSH-Px活性也显著增加（P≤0.01）。从本研究可以得出结论，补充这些维生素有助于将乳汁中改变的氧化剂/抗氧化剂平衡改善至正常状态，从而使印度水牛从亚临床乳腺炎中恢复。
