Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín, CSIC, Apartado 419, E-18008 Granada, Spain.
Environ Pollut. 2012 Jul;166:136-43. doi: 10.1016/j.envpol.2012.03.012. Epub 2012 Apr 11.
Environmental contamination by arsenic constitutes a problem in many countries, and its accumulation in food crops may pose health complications for humans. Reactive oxygen species (ROS) and reactive nitrogen species (RNS) are involved at various levels in the mechanism of responding to environmental stress in higher plants. Using Arabidopsis seedlings exposed to different arsenate concentrations, physiological and biochemical parameters were analyzed to determine the status of ROS and RNS metabolisms. Arsenate provoked a significant reduction in growth parameters and an increase in lipid oxidation. These changes were accompanied by an alteration in antioxidative enzymes and the nitric oxide (NO) metabolism, with a significant increase in NO content, S-nitrosoglutathione reductase (GSNOR) activity and protein tyrosine nitration as well as a concomitant reduction in glutathione and S-nitrosoglutathione (GSNO) content. Our results indicate that 500 μM arsenate (AsV) causes nitro-oxidative stress in Arabidopsis, being the glutathione reductase and the GSNOR activities clearly affected.
砷对环境的污染在许多国家都是一个问题，其在粮食作物中的积累可能会给人类健康带来并发症。活性氧（ROS）和活性氮（RNS）在高等植物应对环境胁迫的机制中在各个层面上都有涉及。使用暴露在不同砷酸盐浓度下的拟南芥幼苗，分析生理和生化参数以确定 ROS 和 RNS 代谢的状况。砷酸盐会导致生长参数显著降低和脂质氧化增加。这些变化伴随着抗氧化酶和一氧化氮（NO）代谢的改变，NO 含量、S-亚硝基谷胱甘肽还原酶（GSNOR）活性和蛋白质酪氨酸硝化显著增加，同时谷胱甘肽和 S-亚硝基谷胱甘肽（GSNO）含量减少。我们的结果表明，500μM 的砷酸盐（AsV）会导致拟南芥的硝态-氧化应激，谷胱甘肽还原酶和 GSNOR 活性明显受到影响。
