Centre of Biological Engineering, Institute for Biotechnology and Bioengineering IBB, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
Bioprocess Biosyst Eng. 2013 Sep;36(9):1267-75. doi: 10.1007/s00449-012-0871-5. Epub 2012 Dec 14.
Pichia pastoris CBS 2612 behavior under air pressures of 1, 3 and 5 bar in culture media of glycerol (pure and crude) and methanol was studied. Generally, the increase in oxygen transfer rate due to the increase of total pressure improved cellular growth for all carbon sources and for batch and fed-batch processes with different feeding rate strategies. In batch cultures, 1.4-, 1.2-, and 1.5-fold improvement in biomass production was obtained with the increase of air pressure up to 5 bar, using methanol, pure glycerol, and crude glycerol, respectively. The increase of air pressure to 5 bar using exponential feeding rate led to 1.4-fold improvement in biomass yield per glycerol mass consumed, for crude and pure glycerol. The current low cost of crude glycerol from the biodiesel production together with the present results shows the possibility of improving cell mass production of P. pastoris using increased air pressure.
研究了毕赤酵母 CBS 2612 在甘油（纯甘油和粗甘油）和甲醇培养基中，在 1、3 和 5 巴的空气压力下的行为。通常，由于总压力的增加而导致的氧传递速率的增加改善了所有碳源的细胞生长，以及使用不同进料速率策略的分批和补料分批工艺。在分批培养中，使用甲醇、纯甘油和粗甘油，将空气压力增加到 5 巴分别使生物量的生产提高了 1.4 倍、1.2 倍和 1.5 倍。使用指数进料速率将空气压力增加到 5 巴，使粗甘油和纯甘油的每消耗甘油质量的生物量产率提高了 1.4 倍。目前生物柴油生产中粗甘油的低成本以及目前的结果表明，使用增加的空气压力可以提高毕赤酵母细胞质量的生产。
