Centro de Biotecnologia Molecular, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF 70910-900, Brazil.
Laboratório de Materiais e Combustíveis, Instituto de Química, Universidade de Brasília, Brasília, DF 70910-000, Brazil.
Bioresour Technol. 2014;152:505-10. doi: 10.1016/j.biortech.2013.11.042. Epub 2013 Nov 27.
Crude glycerol, also known as glycerin, is the main byproduct of the biodiesel industry. It has been estimated that up to 40,000 tons of glycerin will be produced each year by 2020. This study evaluated the value-added use of crude glycerol derived from soybean biodiesel preparation as a carbon source for heterologous protein production using the yeast Pichia pastoris. Eleven glycerin samples were obtained by methanolysis of soybean oil using different acids or bases as catalysts. Cell growth experiments showed that crude glycerol containing either potassium or sodium hydroxide resulted in 1.5-2 times higher final cell densities when compared to glycerol P.A. Finally, crude glycerol containing sodium hydroxide was successfully utilized for constitutive heterologous α-amylase production in P. pastoris. This study demonstrated that crude glycerol without any purification steps may be directly used as carbon source for protein production in P. pastoris.
粗甘油，也称为甘油，是生物柴油工业的主要副产物。据估计，到 2020 年，每年将生产多达 40000 吨的甘油。本研究评估了从大豆生物柴油制备中获得的粗甘油作为酵母毕赤酵母异源蛋白生产的碳源的增值利用。通过使用不同的酸或碱作为催化剂对大豆油进行甲醇解，得到了 11 种甘油样品。细胞生长实验表明，与甘油 P.A.相比，含有氢氧化钾或氢氧化钠的粗甘油最终细胞密度高 1.5-2 倍。最后，氢氧化钠的粗甘油成功地用于毕赤酵母中组成型异源α-淀粉酶的生产。本研究表明，无需任何纯化步骤的粗甘油可直接用作毕赤酵母中蛋白质生产的碳源。
