Department of Biology and Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
Chembiochem. 2013 Jan 2;14(1):53-7. doi: 10.1002/cbic.201200700. Epub 2012 Dec 13.
Caught in the act: The FRET-Capture approach exploits a bound solvatochromic fluorophore, 4-N,N-dimethylamino-1,8-naphthalimide, as a FRET donor in both inter- and intramolecular energy transfer. A unique feature of this method is the additional level of signal selectivity as the FRET signal is only turned on when the donor is specifically bound to the protein of interest, eliminating high background and false positive signals.
FRET-Capture 方法利用结合的溶致变色荧光团 4-N,N-二甲基氨基-1,8-萘酰亚胺作为 FRET 供体，用于分子间和分子内能量转移。该方法的一个独特特点是信号选择性的额外水平，因为只有当供体特异性结合到感兴趣的蛋白质时，FRET 信号才会打开，从而消除高背景和假阳性信号。
