Henderson D E, Mello J A
Department of Chemistry, Trinity College, Hartford, CT 06106.
J Chromatogr. 1990 Jan 19;499:79-88. doi: 10.1016/s0021-9673(00)96965-6.
The high-performance liquid chromatographic separation of biologically active peptides containing 1-prolyl residues is demonstrated. Rapid isomer interconversion between cis- and trans-isomeric forms at the prolyl peptide bond is shown to produce classical secondary equilibrium effects in the peak shapes. By operating the column at temperatures in the range -15 degrees C to 5 degrees C it is possible to obtain normal separations of the various isomeric forms. The use of low column temperatures makes physicochemical studies possible, e.g., the effect of pH on isomer composition in free solution as reported here for morphiceptin.
本文展示了含1-脯氨酰残基的生物活性肽的高效液相色谱分离。结果表明，脯氨酰肽键处顺式和反式异构体形式之间的快速异构体相互转化在峰形上产生了典型的二级平衡效应。通过在-15℃至5℃的温度范围内操作色谱柱，可以实现各种异构体形式的正常分离。使用低柱温使得物理化学研究成为可能，例如本文报道的吗啡肽在游离溶液中pH对异构体组成的影响。
