Mabuchi H, Nakahashi H
J Chromatogr. 1981 Aug 28;213(2):275-86. doi: 10.1016/s0021-9673(00)81910-x.
The systematic separation of medium-sized biologically active peptides by high-performance liquid chromatography (HPLC) is described. Three steps are involved: first, high-performance sodium dodecyl sulphate (SDS) gel chromatography on a newly developed column. TSK-GEL 2000SW; secondly, ion-pair reversed-phase HPLC using stepwise elution mobile phases containing SDS and tetrabutylammonium phosphate; thirdly, high-performance cation-exchange chromatography on a Partisil SCX column for purification, using stepwise gradient elution with volatile buffers. Removal of SDS was possible in the final step. This systematic method is fast, reproducible and gives excellent separations and recoveries.
描述了通过高效液相色谱法（HPLC）对中等大小生物活性肽进行系统分离的方法。该方法包括三个步骤：首先，在新开发的TSK-GEL 2000SW柱上进行高效十二烷基硫酸钠（SDS）凝胶色谱；其次，使用含有SDS和磷酸四丁铵的梯度洗脱流动相进行离子对反相HPLC；第三，在Partisil SCX柱上进行高效阳离子交换色谱纯化，使用挥发性缓冲液进行梯度洗脱。最后一步可以去除SDS。这种系统方法快速、可重复，分离效果和回收率都非常好。
