Department of Animal Sciences, Ohio Agricultural Research and Development Center, Wooster 44691, USA.
Poult Sci. 2013 Jan;92(1):138-42. doi: 10.3382/ps.2012-02593.
The CD4(+)CD25(+) cells have T regulatory cell properties in chickens. This study investigated the effect of in ovo injection of anti-chicken CD25 monoclonal antibodies (0.5 mg/egg) on CD4(+)CD25(+) cell depletion and on amounts of interleukin-2 mRNA and interferon-γ mRNA in CD4(+)CD25(-) cells posthatch. Anti-chicken CD25 or PBS (control) was injected into 16-d-old embryos. Chicks hatched from eggs injected with anti-chicken CD25 antibodies had a lower CD4(+)CD25(+) cell percentage in the blood until 25 d posthatch. The anti-chicken CD25 antibody injection nearly depleted CD4(+)CD25(+) cells in the blood until 16 d posthatch. At 30 d posthatch, the CD4(+)CD25(+) cell percentage in the anti-CD25-antibody-injected group was comparable with the percentage in the control group. At 16 d posthatch, the anti-chicken CD25 antibody injection decreased CD4(+)CD25(+) cell percentages in the thymus, spleen, and cecal tonsils. Chickens hatched from anti-CD25-antibody-injected eggs had approximately 25% of CD4(+)CD25(+) cells in the cecal tonsils and thymus compared with those in the cecal tonsils and thymus of the control group. The CD4(+)CD25(-) cells from the spleen and cecal tonsils of chicks hatched from anti-chicken-CD25-injected eggs had higher amounts of interferon-γ and interleukin-2 mRNA than CD4(+)CD25(-) cells from the control group. It could be concluded that injecting anti-chicken CD25 antibodies in ovo at 16 d of incubation nearly depleted the CD4(+)CD25(+) cells until 25 d posthatch.
鸡的 CD4(+)CD25(+)细胞具有 T 调节细胞特性。本研究探讨了在鸡胚期注射抗鸡 CD25 单克隆抗体（0.5mg/ 枚）对 CD4(+)CD25(+)细胞耗竭的影响，以及对孵化后 CD4(+)CD25(-)细胞中白细胞介素 2 mRNA 和干扰素-γ mRNA 含量的影响。在 16 日龄胚胎中注射抗鸡 CD25 或 PBS（对照）。从注射抗鸡 CD25 抗体的鸡蛋中孵化的小鸡在孵化后 25 天内血液中的 CD4(+)CD25(+)细胞比例较低。抗鸡 CD25 抗体注射几乎耗尽了血液中的 CD4(+)CD25(+)细胞，直到孵化后 16 天。孵化后 30 天，抗 CD25 抗体注射组的 CD4(+)CD25(+)细胞百分比与对照组相当。孵化后 16 天，抗鸡 CD25 抗体注射降低了胸腺、脾脏和盲肠扁桃体中 CD4(+)CD25(+)细胞的比例。从抗鸡 CD25 抗体注射鸡蛋中孵化的小鸡盲肠扁桃体和胸腺中的 CD4(+)CD25(+)细胞百分比约为对照组盲肠扁桃体和胸腺中的 25%。从抗鸡 CD25 抗体注射鸡蛋中孵化的小鸡脾脏和盲肠扁桃体的 CD4(+)CD25(-)细胞中干扰素-γ和白细胞介素 2 mRNA 的含量高于对照组。可以得出结论，在孵化 16 天时向鸡胚中注射抗鸡 CD25 抗体几乎可以耗尽 CD4(+)CD25(+)细胞，直到孵化后 25 天。
