Department of Poultry Science, Auburn University, Auburn, AL 38649, USA.
Poult Sci. 2013 Jan;92(1):158-70. doi: 10.3382/ps.2012-02365.
Two identical trials were conducted to determine the relationship of a novel digestive enzyme assay, Poultry Complete IDEA (PC IDEA), and the pepsin digestibility assay with standardized ileal amino acid digestibility (SIAAD) of 20 animal protein meals (APM) fed to broilers from 25 to 30 d of age. Animal protein meals included 10 meat and bone meals (MBM) consisting of bovine, porcine, or mixed bovine and porcine raw materials (BP), and 10 animal protein blends containing animal proteins from various species. Treatments consisted of 20 semi-purified diets containing 1 APM as the sole source of dietary amino acids (AA), and 1 N-free diet to determine endogenous ileal AA flow. With the exception of the N-free diet, diets were formulated to contain 20% CP. In each trial, 756 Ross × Ross 708 male broilers were housed in battery cages and randomly assigned to 21 dietary treatments on d 25 (12 birds per cage; 3 replicate cages), and ileal digesta were collected on d 30 for determination of SIAAD. Pepsin digestibility and PC IDEA were determined for APM samples from each experimental diet (3 replicates per trial; 6 total replicates). Pepsin digestibility and PC IDEA were both correlated (P < 0.001) with SIAAD for each AA. Multiple linear regression of PC IDEA and pepsin digestibility on SIAAD resulted in the following equations: % Lys SIAAD = [-9.65 + (0.38 × % PC IDEA predicted Lys digestibility) + (0.69 × % pepsin digestibility)], % Met SIAAD = [-35.95 + (0.62 × % PC IDEA predicted Met digestibility) + (0.75 × % pepsin digestibility)], % Thr SIAAD = [-77.5 + (0.39 × % PC IDEA predicted Thr digestibility) + (1.37 × % pepsin digestibility)]. Values of R(2) were 0.46, 0.47, and 0.55 for Lys, Met, and Thr, respectively. The relatively low R(2) values may have been due to the limited range in SIAAD observed for the 20 APM, and additional data on APM varying in SIAAD are needed.
进行了两项相同的试验，以确定一种新型消化酶测定法——家禽综合 IDEA（PC IDEA）和胃蛋白酶消化率测定法与 20 种动物蛋白饲料（APM）在 25 至 30 日龄肉鸡中的标准回肠氨基酸消化率（SIAAD）之间的关系。动物蛋白饲料包括 10 种肉骨粉（MBM），其原料由牛、猪或牛猪混合组成（BP），以及 10 种含有各种来源动物蛋白的动物蛋白混合物。处理组由 20 种半纯化日粮组成，其中 1 种 APM 作为日粮氨基酸（AA）的唯一来源，1 种无氮日粮用于确定回肠内源性 AA 流量。除无氮日粮外，日粮的 CP 含量均为 20%。在每个试验中，756 只 Ross×Ross 708 雄性肉鸡饲养在笼中，在第 25 天（每个笼 12 只鸡；3 个重复笼）随机分配到 21 种日粮处理组，并在第 30 天收集回肠内容物以确定 SIAAD。对每个试验的实验日粮中的 APM 样品进行胃蛋白酶消化率和 PC IDEA 测定（每个试验 3 个重复；总共 6 个重复）。胃蛋白酶消化率和 PC IDEA 均与每个 AA 的 SIAAD 呈正相关（P<0.001）。PC IDEA 和胃蛋白酶消化率对 SIAAD 的多元线性回归得出以下方程：%Lys SIAAD=[-9.65+（0.38×%PC IDEA 预测 Lys 消化率）+（0.69×%胃蛋白酶消化率）]，%Met SIAAD=[-35.95+（0.62×%PC IDEA 预测 Met 消化率）+（0.75×%胃蛋白酶消化率）]，%Thr SIAAD=[-77.5+（0.39×%PC IDEA 预测 Thr 消化率）+（1.37×%胃蛋白酶消化率）]。Lys、Met 和 Thr 的 R2 值分别为 0.46、0.47 和 0.55。R2 值相对较低可能是由于 20 种 APM 观察到的 SIAAD 范围有限，需要更多关于 SIAAD 变化的 APM 数据。
