Department of Animal and Food Sciences, University of Kentucky, Lexington KY, USA.
Alltech Inc., Catnip Pike, Nicholasville KY, USA.
Poult Sci. 2018 Jan 1;97(1):131-139. doi: 10.3382/ps/pex271.
Two studies were conducted to evaluate the effect of dietary Ca levels (low, 1% and high, 3%) on ileal endogenous amino acid losses (IEAAL) and standardized ileal amino acid digestibility (SIAAD) in broilers (BR) and laying hens (LH) fed nitrogen-free diets (NFD) and distiller's dried grain with solubles (DDGS)-based diets. A total of 384 male Cobb 500 BR and 288 LH were used in a completely randomized design (CRD) with 16 (BR) or 12 (LH) replicate cages with 6 birds/replicate. IEAAL and apparent ileal digestibility (AID) of AA were analyzed using the GLM procedure of SAS appropriate for a CRD while SIAAD values were analyzed using the GLM procedure of SAS appropriate for a 2 × 2 factorial arrangement of treatments. For BR, IEAAL and N losses (mg/kg of dry matter intake, DMI) were higher (P < 0.05) when NFD with high Ca level was fed (total AA was 39%, N was 35% higher). For most of the AA, AID was higher (P < 0.05) in BR fed DDGS-based diet with high Ca level. High dietary Ca resulted in higher (P < 0.05) SIAAD for all the AA except for Arg, Lys, Met, Cys, and Tyr. For LH, AID of AA was higher (P < 0.05) for the DDGS diet with high Ca level in 13 of the 18 AA evaluated. There was interaction (P < 0.05) between diet Ca level and correction method on LH SIAAD values for Thr, Asp, Gly, and Ser. The SIAAD values for 8 AA were higher (P < 0.05) in birds on high Ca DDGS diet. Correction with low Ca NFD resulted in higher (P < 0.05) SIAAD values for all the AA. Result from this study showed that high Ca increased total IEAAL in BR by 39% but decreased same by 27% in LH. Finally, SIAAD values were increased in BR fed high Ca DDGS-based diet while SIAAD value in LH was lower when correction was done using values from high Ca-NFD fed birds.
本研究开展了两项试验，以评估饲粮钙水平（低钙：1%，高钙：3%）对以无氮饲粮（NFD）和玉米DDGS 饲粮饲养的肉鸡（BR）和产蛋鸡（LH）回肠内源氨基酸损失（IEAAL）和回肠氨基酸表观消化率（AID）的影响。采用完全随机设计（CRD），将 384 只雄性科宝 500BR 和 288 只 LH 分别饲养于 16（BR）或 12（LH）个重复笼中，每个重复 6 只鸡。使用 SAS 中的 GLM 程序分析 IEAAL 和 AA 的 AID，该程序适用于 CRD；使用 SAS 中的 GLM 程序分析 SIAAD 值，该程序适用于 2×2 处理因素的完全析因设计。对于 BR，高钙 NFD 组的 IEAAL 和氮损失（mg/kg 干物质采食量，DMI）更高（P<0.05）（总 AA 增加 39%，氮增加 35%）。对于大多数 AA，高钙 DDGS 饲粮组的 BR 的 AID 更高（P<0.05）。高钙饲粮显著提高了除 Arg、Lys、Met、Cys 和 Tyr 外的所有 AA 的 SIAAD 值。对于 LH，在 18 种 AA 中，有 13 种 AA 的高钙 DDGS 饲粮组的 AID 更高（P<0.05）。饲粮钙水平和校正方法对 LH 的 Thr、Asp、Gly 和 Ser 的 SIAAD 值存在互作（P<0.05）。8 种 AA 的 SIAAD 值在高钙 DDGS 饲粮组的鸡中更高（P<0.05）。用低钙 NFD 校正后，所有 AA 的 SIAAD 值都更高（P<0.05）。本研究表明，高钙使 BR 的总 IEAAL 增加了 39%，但使 LH 的 IEAAL 减少了 27%。最后，高钙 DDGS 饲粮显著提高了 BR 的 SIAAD 值，而用高钙 NFD 校正后，LH 的 SIAAD 值降低。
