Department of Public Health Sciences, School of Public Health, University of Alberta, Edmonton, Alberta, Canada.
Ann Allergy Asthma Immunol. 2013 Jan;110(1):44-50.e1. doi: 10.1016/j.anai.2012.11.003.
Workers in swine operations are exposed to indoor dusts and gases and are at increased risk of respiratory problems. Toll-like receptor (TLR) 2 recognizes ligands from gram-positive bacteria, whereas TLR4 responds to endotoxin from gram-negative bacteria.
To investigate the effects of TLR2 and TLR4 polymorphisms on lung function in workers from swine operations and nonfarming rural dwellers.
A total of 374 full-time workers from large swine operations and 411 nonfarming rural dwellers from Saskatchewan were included. Information on demography, lifestyle, and occupation, lung function measurements, and blood samples for genotyping were obtained from the participants. Multiple regression analysis and Bonferroni correction were used in the statistical analysis.
Workers with TLR2-16933T/A polymorphism (AA) had significantly greater mean values of lung function than workers with wild-type genotypes (AT+TT) after controlling for potential confounders (forced expiratory volume in 1 second, 3.7 vs 3.5 L; P=.009; forced expiratory flow between 25% and 75%, 3.7 vs 3.3 L; P=.003; predicted forced expiratory volume in 1 second; 100.3% vs 95.6%; P=.005; forced expiratory flow between 25% and 75%, 92.4% vs 83.4%; P=.009). These results were also observed for TLR2Arg677Trp polymorphism among the workers. No such significant differences were observed among nonfarming rural dwellers. For Asp299Gly and Thr399Ile polymorphisms in the TLR4 gene, no significant differences were observed in the mean lung function values between the polymorphic and wild-type groups in both workers and rural dwellers.
Our study is the first, to our knowledge, to report protective effects of TLR2 polymorphisms on lung function among workers in swine operations and raises the possibility that TLR2 polymorphisms are protective of airway disease in individuals exposed to gram-positive organisms in the inhaled airborne dust.
从事养猪作业的工人会接触室内灰尘和气体，患呼吸道问题的风险增加。Toll 样受体（TLR）2 识别革兰氏阳性菌的配体，而 TLR4 则对革兰氏阴性菌的内毒素作出反应。
研究 TLR2 和 TLR4 多态性对养猪作业工人和非农业农村居民肺功能的影响。
共纳入来自萨斯喀彻温省的 374 名大型养猪场全职工人和 411 名非农业农村居民。从参与者那里获得了人口统计学、生活方式和职业信息、肺功能测量值和用于基因分型的血样。在统计分析中使用了多元回归分析和 Bonferroni 校正。
在控制了潜在的混杂因素后，TLR2-16933T/A 多态性（AA）的工人的肺功能平均值明显大于野生型基因型（AT+TT）的工人（1 秒用力呼气量，3.7 比 3.5 L；P=.009；25%至 75%用力呼气流量，3.7 比 3.3 L；P=.003；预计 1 秒用力呼气量；100.3%比 95.6%；P=.005；25%至 75%之间的用力呼气流量，92.4%比 83.4%；P=.009）。在工人中，TLR2Arg677Trp 多态性也观察到了同样的结果。在非农业农村居民中，没有观察到这种显著差异。对于 TLR4 基因中的 Asp299Gly 和 Thr399Ile 多态性，在工人和农村居民中，多态性和野生型组之间的平均肺功能值没有显著差异。
我们的研究首次报道了 TLR2 多态性对养猪作业工人肺功能的保护作用，并提出了 TLR2 多态性可能对吸入空气中的革兰氏阳性菌引起的气道疾病具有保护作用的可能性。
