Department of Molecular and Cellular Physiology, Stanford Institute for Neuro-Innovation and Translational Neurosciences, Stanford Medical School, Stanford, California 94305, USA.
Annu Rev Physiol. 2013;75:393-422. doi: 10.1146/annurev-physiol-020911-153305.
Regulated exocytosis and endocytosis are critical to the function of many intercellular networks, particularly the complex neural circuits underlying mammalian behavior. Kiss-and-run (KR) is an unconventional fusion between secretory vesicles and a target membrane that releases intravesicular content through a transient, nanometer-sized fusion pore. The fusing vesicle retains its gross shape, precluding full integration into the planar membrane, and enough molecular components for rapid retrieval, reacidification, and reuse. KR makes judicious use of finite presynaptic resources, and mounting evidence suggests that it influences synaptic information transfer. Here we detail emerging perspectives on KR and its role in neurotransmission. We additionally formulate a restraining force hypothesis as a plausible mechanistic basis for KR and its physiological modulation in small nerve terminals. Clarification of the mechanism and function of KR has bearing on understanding the kinetic transitions underlying SNARE-mediated fusion, interactions between vesicles and their local environment, and the influence of release dynamics on neural information processing.
受调控的胞吐作用和胞吞作用对于许多细胞间网络的功能至关重要，尤其是对于哺乳动物行为所依赖的复杂神经回路。kiss-and-run（KR）是一种独特的分泌囊泡与靶膜之间的融合方式，通过短暂的、纳米级大小的融合孔释放囊泡内物质。融合的囊泡保持其大致形状，无法完全整合到平面膜中，并且存在足够的分子成分，以便快速回收、再酸化和再利用。KR 明智地利用有限的突触前资源，越来越多的证据表明它影响突触信息传递。在这里，我们详细介绍了 KR 的最新观点及其在神经递质传递中的作用。我们还提出了一个约束力量假说，作为 KR 及其在小神经末梢中的生理调节的合理机制基础。阐明 KR 的机制和功能对于理解 SNARE 介导的融合的动力学转变、囊泡与其局部环境之间的相互作用以及释放动力学对神经信息处理的影响具有重要意义。
