College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea.
Food Chem Toxicol. 2013 May;55:241-7. doi: 10.1016/j.fct.2012.12.007. Epub 2012 Dec 14.
Angelica dahurica is used in functional foods for the prevention and treatment of various diseases, such as inflammation and cancer. In the present study, we examined the effect of A. dahurica polysaccharide (ADP) on dendritic cell (DC) maturation. ADP increased the expressions of CD86 and MHC-II molecules, the production of IL-12, IL-1β, and TNF-α, and allogeneic T cell activation ability of DCs, and reduced DC endocytosis. As a mechanism of action, the knockdown of TLR4 with small interfering RNA decreased the ADP-induced production of nitric oxide and IL-12 by DCs, suggesting the membrane receptor candidate of ADP. After binding to TLR4, ADP increased the phosphorylation of ERK, JNK, and p38 MAPKs, and the nuclear translocation of NF-κB p50/p65. These results indicate that ADP activates DCs through TLR4 and downstream signalings.
白芷被用于功能性食品，以预防和治疗各种疾病，如炎症和癌症。在本研究中，我们研究了白芷多糖（ADP）对树突状细胞（DC）成熟的影响。ADP 增加了 CD86 和 MHC-II 分子的表达、IL-12、IL-1β 和 TNF-α 的产生，以及 DC 的同种异体 T 细胞激活能力，并减少了 DC 的内吞作用。作为作用机制，用小干扰 RNA 敲低 TLR4 可降低 ADP 诱导的 DC 产生的一氧化氮和 IL-12，提示 ADP 的膜受体候选物。ADP 与 TLR4 结合后，可增加 ERK、JNK 和 p38 MAPKs 的磷酸化，以及 NF-κB p50/p65 的核易位。这些结果表明 ADP 通过 TLR4 和下游信号通路激活 DC。
