IL-33 促进体内嗜酸性粒细胞增多，并拮抗 IL-5 依赖性嗜酸性粒细胞生成。
IL-33 promotes eosinophilia in vivo and antagonizes IL-5-dependent eosinophil hematopoiesis ex vivo.
机构信息
Inflammation Immunobiology Section of the Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-1883, USA.
出版信息
Immunol Lett. 2013 Feb;150(1-2):41-7. doi: 10.1016/j.imlet.2012.12.002. Epub 2012 Dec 11.
IL-33 is an IL-1 family cytokine that elicits IL-5-dependent eosinophilia in vivo. We show here that IL-33 promotes minimal eosinophil hematopoiesis via direct interactions with mouse bone marrow progenitors ex vivo and that it antagonizes eosinophil hematopoiesis promoted by IL-5 on SCF and Flt3L primed bone marrow progenitor cells in culture. SCF and Flt3L primed progenitors respond to IL-33 by acquiring an adherent, macrophage-like phenotype, and by releasing macrophage-associated cytokines into the culture medium. IL-33-mediated antagonism of IL-5 was reproduced in part by the addition of GM-CSF and was inhibited by the actions of neutralizing anti-GM-CSF antibody. These findings suggest that the direct actions of IL-33 on bone marrow progenitors primed with SCF and Flt3L are antagonistic to the actions of IL-5 and are mediated in part by GM-CSF.
IL-33 是一种白细胞介素 1 家族细胞因子，可在体内诱导致敏嗜酸性粒细胞增多。我们在此表明，IL-33 通过与体外小鼠骨髓祖细胞的直接相互作用，促进最小的嗜酸性粒细胞造血，并且在体外培养中拮抗 IL-5 在 SCF 和 Flt3L 引发的骨髓祖细胞上促进的嗜酸性粒细胞造血。SCF 和 Flt3L 引发的祖细胞通过获得黏附的巨噬细胞样表型并将与巨噬细胞相关的细胞因子释放到培养基中对 IL-33 作出反应。IL-33 介导的对 IL-5 的拮抗作用部分是通过添加 GM-CSF 来再现的，并且被中和抗 GM-CSF 抗体的作用所抑制。这些发现表明，IL-33 对用 SCF 和 Flt3L 引发的骨髓祖细胞的直接作用与 IL-5 的作用相拮抗，并且部分是通过 GM-CSF 介导的。
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