经 RISUG 血管内避孕及其通过 DMSO 和 NaHCO₃ 的逆转后，Wistar 白化大鼠白细胞和睪丸的遗传毒性评价。

Evaluation of genotoxicity in leukocytes and testis following intra-vasal contraception with RISUG and its reversal by DMSO and NaHCO₃ in Wistar albino rats.

机构信息

Centre for Advanced Studies, Department of Zoology, University of Rajasthan, Jaipur 302004, India.

出版信息

Reprod Toxicol. 2013 Apr;36:53-9. doi: 10.1016/j.reprotox.2012.11.005. Epub 2012 Dec 13.

DOI:10.1016/j.reprotox.2012.11.005

Abstract

Evaluation of genotoxicity of RISUG® - a vas based contraceptive, was carried out in the present study. Animals were allotted into groups of sham operated control, vas occlusion with RISUG (5-7 μl) for 360 days and reversal by DMSO (250-500 μl) and 5% NaHCO₃ (500 μl). Blood samples and testis were collected at 360 days of vas occlusion and 90 days of vas occlusion reversal for comet analysis. Hydrogen peroxide induced samples were used as positive control. Olive moment, tail length and percentage DNA in tail were recorded with minimum variation in all groups for both leukocytes and testis. When compared with positive control the variation was highly significant for both 20 μM and 50 μM H₂O₂ (p<0.001). It is concluded that vas occlusion with RISUG at the contraceptive dose regimen is not associated with genotoxicity in leukocytes or the testis of pre- and post-reversal rats.

摘要

本研究旨在评估 RISUG®——一种基于血管的避孕方法——的遗传毒性。动物被分为假手术对照组、RISUG（5-7μl）闭塞血管 360 天组和用 DMSO（250-500μl）和 5%NaHCO₃（500μl）逆转组。在血管闭塞 360 天和血管闭塞逆转 90 天时，收集血液样本和睾丸进行彗星分析。用过氧化氢诱导的样本作为阳性对照。用白细胞和睾丸的最小变异记录了彗星实验中的橄榄矩、尾部长度和尾部 DNA 百分比。与阳性对照组相比，20μM 和 50μM H₂O₂的变化均具有高度显著性（p<0.001）。结论是，在避孕剂量方案下，用 RISUG 闭塞血管不会导致白细胞或逆转前后大鼠睾丸的遗传毒性。