Salam Ansari Abdul, Sevliya Kiran, Badar Ayesha, Lohiya Nirmal Kumar
Department of Zoology, Center for Advanced Studies, University of Rajasthan, Jaipur, Rajasthan, India.
Int J Reprod Biomed. 2021 Jan 25;19(1):47-56. doi: 10.18502/ijrm.v19i1.8179. eCollection 2021 Jan.
Reversible inhibition of sperm under guidance ("RISUGⓇ") is a promising intravasal male contraceptive.
An exploratory study was conducted with a concept of non-invasive, transcervical, single-intervention and reversible contraception using RISUGⓇ in females.
In this experimental study, 60 adult Wistar albino female rats weighing 150-155 g, 3-4 months old were divided into four groups: group I: sham-operated control; group II: tubal occlusion with RISUG for 90 days; group III: tubal occlusion with RISUGⓇ for 90 days and reversal with dimethyl sulphoxide and group IV: tubal occlusion with RISUGⓇ for 90 days and reversal with 5% NaHCO. Animals were subjected to bilateral fallopian tube occlusion with RISUGⓇ and reversal with DMSO and NaHCO . The estrous cycle, fertility and histology of fallopian tube were evaluated.
Group I showed 100% fertility during all mating schedules. Animals of experimental groups indicated positive mating, but 0% fertility was evident following 30, 60, and 90 days of tubal occlusion. However, after reversal, fertility steadily increased to normalcy in groups III (50% at 45 days, 80% at 105 days, 100% at 150 and 195 days) and IV (70% at 45 and 105 days, 100% at 150 and 195 days) animals. Group II illustrated disorganized inner cell linings and eosinated RISUGⓇimplant-filled lumen. Reversal groups (III and IV) revealed complete restoration of cellular histo-architecture. Regular estrous cycle was noticed in all experimental groups.
RISUGⓇ is suitable for single intervention, intratubular, reversible contraception in female rats.
可逆性精子导向抑制法（“RISUGⓇ”）是一种很有前景的输精管内男性避孕方法。
采用RISUGⓇ对雌性动物进行非侵入性、经宫颈、单次干预且可逆的避孕概念进行探索性研究。
在本实验研究中，将60只体重150 - 155克、3 - 4月龄的成年Wistar白化雌性大鼠分为四组：第一组：假手术对照组；第二组：用RISUG进行输卵管阻塞90天；第三组：用RISUGⓇ进行输卵管阻塞90天，然后用二甲基亚砜进行逆转；第四组：用RISUGⓇ进行输卵管阻塞90天，然后用5%碳酸氢钠进行逆转。对动物进行双侧输卵管用RISUGⓇ阻塞，并用二甲基亚砜和碳酸氢钠进行逆转。评估动情周期、生育能力及输卵管组织学情况。
第一组在所有交配期的生育能力均为100%。实验组动物交配呈阳性，但输卵管阻塞30、60和90天后的生育能力为0%。然而，逆转后，第三组（45天时为50%，105天时为80%，150和195天时为100%）和第四组（45和105天时为70%，150和195天时为100%）动物的生育能力稳步恢复至正常。第二组显示内部细胞内衬紊乱，管腔内充满嗜酸性RISUGⓇ植入物。逆转组（第三组和第四组）显示细胞组织架构完全恢复。所有实验组均观察到规律的动情周期。
RISUGⓇ适用于雌性大鼠的单次干预、输卵管内、可逆性避孕。
