计算确定 DNA-PKcs 的热重复样结构域的取向。
Computational determination of the orientation of a heat repeat-like domain of DNA-PKcs.
机构信息
Department of Chemistry, Vanderbilt University, Nashville, TN 37212, USA.
出版信息
Comput Biol Chem. 2013 Feb;42:1-4. doi: 10.1016/j.compbiolchem.2012.11.001. Epub 2012 Nov 19.
Abstract
DNA dependent protein kinase catalytic subunit (DNA-PKcs) is an important regulatory protein in non-homologous end joining a process used to repair DNA double strand breaks. Medium resolution structures both from cryoEM and X-ray crystallography show the general topology of the protein and positions of helices in parts of DNA-PKcs. EM-Fold, an algorithm developed for building protein models into medium resolution density maps has been used to generate models for the heat repeat-like "Ring structure" of the molecule. We were able to computationally corroborate placement of the N-terminus of the domain that supports a previously published hypothesis. Targeted experiments are suggested to test the model.
摘要
DNA 依赖性蛋白激酶催化亚基（DNA-PKcs）是一种在非同源末端连接过程中起重要调节作用的蛋白，该过程用于修复 DNA 双链断裂。低温电子显微镜和 X 射线晶体学的中等分辨率结构都显示了该蛋白的一般拓扑结构和 DNA-PKcs 部分螺旋的位置。EM-Fold 是一种用于将蛋白模型构建到中等分辨率密度图中的算法，该算法已被用于生成分子的热重复样“环结构”模型。我们能够通过计算来证实支持先前发表假说的结构域的 N 端位置。建议进行靶向实验来测试该模型。
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