Cherayil G D
Department of Pathology, Medical College of Wisconsin, Milwaukee 53226.
J Neurol Sci. 1990 Feb;95(2):141-51. doi: 10.1016/0022-510x(90)90237-h.
Heparinized blood from 43 patients with multiple sclerosis (MS) and 43 controls matched for age, sex and race were incubated at 42 degrees C for 24 h. The hematocrit values were determined before and after heating. The plasma and red blood cells (RBC) were separated and the plasma free fatty acids (FFA), total neutral lipids (NL; which included the triglycerides and cholesteryl esters), phospholipids (PL), and RBC lipids from 24 patients and 24 controls were isolated. The total amounts of the various FFA in the plasma and the relative percentage fatty acid compositions of the various FFA, NL, ester-linked fatty acids of plasma PL, RBC lipids as well as those of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) fractions of RBC lipids were determined by gas-liquid chromatography (GLC). The hematocrit values were significantly higher in heated samples from both MS and controls. The results of fatty acid analyses from 24 pairs of heated and unheated MS and controls showed that the concentrations of total FFA and most of the fatty acids increased significantly in the heated plasma of both MS and control samples, compared to the respective unheated plasma samples. When the FFA values were expressed as relative percentage compositions, the results showed significant increases in palmitic, arachidonic and adrenic acids and significant decreases in oleic, linoleic and linolenic acids in the heated samples from MS and controls compared to the corresponding unheated samples. In NL the relative percentages of linoleic and arachidonic acids were significantly higher in the heated samples from both MS and controls, while in the PL, the percentages of linoleic acid were lower in the heated samples from both MS and controls, when compared to the corresponding unheated samples. The fatty acid compositions of the RBC lipids showed highly significant increases in palmitic plus palmitoleic, stearic and oleic acids and significant decreases in linoleic and most of the polyunsaturated acids in the heated MS samples compared to the unheated MS samples. In contrast, the fatty acid compositions of the RBC lipids from heated controls showed significant increase in only palmitic plus palmitoleic acids and significant decreases in linoleic and arachidonic acids, compared to the values from unheated controls. The results show that the RBC membranes from MS were more affected by the heat than those of control RBC, indicating possible differences between blood from MS patients and controls.
采集了43例多发性硬化症（MS）患者以及43例年龄、性别和种族相匹配的对照者的肝素化血液，在42摄氏度下孵育24小时。测定加热前后的血细胞比容值。分离血浆和红细胞（RBC），并从24例患者和24例对照者中分离出血浆游离脂肪酸（FFA）、总中性脂质（NL；包括甘油三酯和胆固醇酯）、磷脂（PL）以及红细胞脂质。通过气液色谱法（GLC）测定血浆中各种FFA的总量以及各种FFA、NL、血浆PL的酯连接脂肪酸、红细胞脂质以及红细胞脂质的磷脂酰胆碱（PC）和磷脂酰乙醇胺（PE）组分的相对脂肪酸组成百分比。MS患者和对照者加热后的样本中血细胞比容值均显著升高。对24对加热和未加热的MS患者及对照者进行脂肪酸分析的结果显示，与各自未加热的血浆样本相比，MS患者和对照者加热后的血浆中总FFA浓度以及大多数脂肪酸浓度均显著增加。当FFA值以相对百分比组成表示时，结果显示与相应未加热样本相比，MS患者和对照者加热后的样本中棕榈酸、花生四烯酸和肾上腺酸显著增加，油酸、亚油酸和亚麻酸显著减少。在NL中，MS患者和对照者加热后的样本中亚油酸和花生四烯酸的相对百分比均显著更高，而在PL中，与相应未加热样本相比，MS患者和对照者加热后的样本中亚油酸百分比更低。与未加热的MS样本相比，加热后的MS样本中红细胞脂质的脂肪酸组成显示棕榈酸加棕榈油酸、硬脂酸和油酸显著增加，亚油酸和大多数多不饱和酸显著减少。相比之下，与未加热的对照者相比，加热后的对照者红细胞脂质的脂肪酸组成仅显示棕榈酸加棕榈油酸显著增加，亚油酸和花生四烯酸显著减少。结果表明，MS患者的红细胞膜比对照者的红细胞膜受加热的影响更大，这表明MS患者血液与对照者血液之间可能存在差异。
