Oakes W J, Friedman H S, Bigner S H, Bullock N H, Bigner D D
Department of Surgery, Duke University Medical Center, Durham, North Carolina.
J Neurosurg. 1990 May;72(5):821-3. doi: 10.3171/jns.1990.72.5.0821.
The development of the Cavitron ultrasonic surgical aspirator (CUSA) has facilitated neurosurgical intervention for removal of central or peripheral nervous system tumors adjacent to or within vital structures. However, laboratory studies defining the phenotypic and genotypic properties of these tumors, both in cell culture and as xenografts in immunoincompetent animals, require viable tumor fragments free of microbial or red blood cell contamination. This report describes the use of a readily available sterile trap with the CUSA which, in conjunction with centrifugation and ammonium chloride lysis of the bloody aspirate, allowed collection of concentrated viable human medulloblastoma tumor cells. These cells were successfully established in cell culture and as transplantable xenografts in athymic mice.
卡维tron超声手术吸引器（CUSA）的发展促进了神经外科手术对重要结构附近或内部的中枢或周围神经系统肿瘤的切除。然而，在细胞培养以及在免疫缺陷动物中作为异种移植来定义这些肿瘤的表型和基因型特性的实验室研究，需要不含微生物或红细胞污染的活肿瘤碎片。本报告描述了将一种易于获得的无菌收集器与CUSA一起使用，该收集器与血性吸出物的离心和氯化铵裂解相结合，使得能够收集浓缩的活人类髓母细胞瘤肿瘤细胞。这些细胞成功地在细胞培养中建立，并在无胸腺小鼠中作为可移植的异种移植瘤。
