Beckner Marie E, Jane Esther P, Jankowitz Brian, Agostino Naomi R, Walter Kevin A, Hamilton Ronald L, Pollack Ian F
Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.
Cancer Lett. 2007 Sep 18;255(1):135-44. doi: 10.1016/j.canlet.2007.04.005. Epub 2007 May 31.
Studies of primary cells from malignant brain tumors such as glioblastomas are limited by the small size of surgically resected specimens. However, glioblastomas are also frequently debulked via ultrasonic aspiration. In this study, we examined the functional competence and growth of their aspirated cells. Cells from minced tissue and aspirations were comparable in migration, formation of pseudopodia, development of cellular spheres with radial outgrowth, and neuroectodermal features. Cultures were maintained for more than six weeks without fibroblastic overgrowth. Our observations show that ultrasonically aspirated specimens contain cells useful for studies of tumor migration and growth of tumorspheres.
对胶质母细胞瘤等恶性脑肿瘤原代细胞的研究受到手术切除标本体积小的限制。然而，胶质母细胞瘤也经常通过超声吸引进行减瘤。在本研究中，我们检测了其吸出细胞的功能活性和生长情况。来自切碎组织和吸出物的细胞在迁移、伪足形成、具有放射状生长的细胞球形成以及神经外胚层特征方面具有可比性。培养物维持了六周以上而没有成纤维细胞过度生长。我们的观察结果表明，超声吸出的标本含有可用于研究肿瘤迁移和肿瘤球生长的细胞。
