胶质母细胞瘤超声抽吸所得肿瘤细胞迁移并形成带有放射状生长的球体。
Tumor cells from ultrasonic aspirations of glioblastomas migrate and form spheres with radial outgrowth.
作者信息
Beckner Marie E, Jane Esther P, Jankowitz Brian, Agostino Naomi R, Walter Kevin A, Hamilton Ronald L, Pollack Ian F
机构信息
Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.
出版信息
Cancer Lett. 2007 Sep 18;255(1):135-44. doi: 10.1016/j.canlet.2007.04.005. Epub 2007 May 31.
Abstract
Studies of primary cells from malignant brain tumors such as glioblastomas are limited by the small size of surgically resected specimens. However, glioblastomas are also frequently debulked via ultrasonic aspiration. In this study, we examined the functional competence and growth of their aspirated cells. Cells from minced tissue and aspirations were comparable in migration, formation of pseudopodia, development of cellular spheres with radial outgrowth, and neuroectodermal features. Cultures were maintained for more than six weeks without fibroblastic overgrowth. Our observations show that ultrasonically aspirated specimens contain cells useful for studies of tumor migration and growth of tumorspheres.
摘要
对胶质母细胞瘤等恶性脑肿瘤原代细胞的研究受到手术切除标本体积小的限制。然而，胶质母细胞瘤也经常通过超声吸引进行减瘤。在本研究中，我们检测了其吸出细胞的功能活性和生长情况。来自切碎组织和吸出物的细胞在迁移、伪足形成、具有放射状生长的细胞球形成以及神经外胚层特征方面具有可比性。培养物维持了六周以上而没有成纤维细胞过度生长。我们的观察结果表明，超声吸出的标本含有可用于研究肿瘤迁移和肿瘤球生长的细胞。
相似文献
1
Tumor cells from ultrasonic aspirations of glioblastomas migrate and form spheres with radial outgrowth.胶质母细胞瘤超声抽吸所得肿瘤细胞迁移并形成带有放射状生长的球体。
Cancer Lett. 2007 Sep 18;255(1):135-44. doi: 10.1016/j.canlet.2007.04.005. Epub 2007 May 31.
2
MiR-148a inhibits the proliferation and migration of glioblastoma by targeting ITGA9.miR-148a 通过靶向 ITGA9 抑制脑胶质瘤的增殖和迁移。
Hum Cell. 2019 Oct;32(4):548-556. doi: 10.1007/s13577-019-00279-9. Epub 2019 Sep 5.
3
Isolation and characterization of human malignant glioma cells from histologically normal brain.从组织学正常的脑组织中分离并鉴定人恶性胶质瘤细胞。
J Neurosurg. 1997 Mar;86(3):525-31. doi: 10.3171/jns.1997.86.3.0525.
4
Contribution of gap junctional communication between tumor cells and astroglia to the invasion of the brain parenchyma by human glioblastomas.肿瘤细胞与星形胶质细胞之间的缝隙连接通讯对人胶质母细胞瘤侵袭脑实质的作用。
BMC Cell Biol. 2005 Feb 16;6(1):7. doi: 10.1186/1471-2121-6-7.
5
CRN2 enhances the invasiveness of glioblastoma cells.CRN2 增强脑胶质母细胞瘤细胞的侵袭性。
Neuro Oncol. 2013 May;15(5):548-61. doi: 10.1093/neuonc/nos388. Epub 2013 Feb 14.
6
Rapid detection of high-level oncogene amplifications in ultrasonic surgical aspirations of brain tumors.快速检测脑肿瘤超声吸引术中的高水平癌基因扩增。
Diagn Pathol. 2012 Jun 12;7:66. doi: 10.1186/1746-1596-7-66.
7
Migration pathways of human glioblastoma cells xenografted into the immunosuppressed rat brain.移植到免疫抑制大鼠脑内的人胶质母细胞瘤细胞的迁移途径
J Neurooncol. 2001 May;52(3):205-15. doi: 10.1023/a:1010620420241.
8
Retinoids inhibit human glioma cell proliferation and migration in primary cell cultures but not in established cell lines.维甲酸在原代细胞培养中可抑制人胶质瘤细胞的增殖和迁移，但在已建立的细胞系中则不然。
Neurosurgery. 2000 Feb;46(2):419-30. doi: 10.1097/00006123-200002000-00029.
9
Effects of EGF, bFGF, NGF and PDGF(bb) on cell proliferative, migratory and invasive capacities of human brain-tumour biopsies in vitro.表皮生长因子（EGF）、碱性成纤维细胞生长因子（bFGF）、神经生长因子（NGF）和血小板衍生生长因子（PDGF - bb）对人脑肿瘤活检组织体外细胞增殖、迁移及侵袭能力的影响
Int J Cancer. 1993 Jan 21;53(2):209-14. doi: 10.1002/ijc.2910530206.
10
Quantitative metrics of net proliferation and invasion link biological aggressiveness assessed by MRI with hypoxia assessed by FMISO-PET in newly diagnosed glioblastomas.在新诊断的胶质母细胞瘤中，净增殖和侵袭的定量指标将通过MRI评估的生物学侵袭性与通过FMISO-PET评估的缺氧联系起来。
Cancer Res. 2009 May 15;69(10):4502-9. doi: 10.1158/0008-5472.CAN-08-3884. Epub 2009 Apr 14.
引用本文的文献
1
Optimized culturing yields high success rates and preserves molecular heterogeneity, enabling personalized screening for high-grade gliomas.优化培养可获得高成功率并保留分子异质性，从而能够对高级别胶质瘤进行个性化筛查。
NPJ Precis Oncol. 2025 May 27;9(1):156. doi: 10.1038/s41698-025-00946-1.
2
Ex vivo ultrasonic samples of human brain tumors in the molecular era.分子时代人脑肿瘤的体外超声样本。
Neurooncol Adv. 2020 Feb 8;2(1):vdaa014. doi: 10.1093/noajnl/vdaa014.
3
Glioma surgical aspirate: a viable source of tumor tissue for experimental research.神经胶质瘤手术吸引物：实验研究中肿瘤组织的可行来源。
Cancers (Basel). 2013 Apr 3;5(2):357-71. doi: 10.3390/cancers5020357.
4
Rapid detection of high-level oncogene amplifications in ultrasonic surgical aspirations of brain tumors.快速检测脑肿瘤超声吸引术中的高水平癌基因扩增。
Diagn Pathol. 2012 Jun 12;7:66. doi: 10.1186/1746-1596-7-66.
5
Dasatinib synergizes with JSI-124 to inhibit growth and migration and induce apoptosis of malignant human glioma cells.达沙替尼与JSI-124协同作用，以抑制恶性人类胶质瘤细胞的生长和迁移并诱导其凋亡。
J Carcinog. 2010 Jul 14;9:7. doi: 10.4103/1477-3163.65448.
6
Identification of ATP citrate lyase as a positive regulator of glycolytic function in glioblastomas.鉴定三磷酸柠檬酸裂解酶为神经胶质瘤中糖酵解功能的正向调节因子。
Int J Cancer. 2010 May 15;126(10):2282-95. doi: 10.1002/ijc.24918.
本文引用的文献
1
Molecular cloning and function of Oct-3 isoforms in cynomolgus monkey embryonic stem cells.食蟹猴胚胎干细胞中Oct-3亚型的分子克隆与功能
Stem Cells Dev. 2006 Aug;15(4):566-74. doi: 10.1089/scd.2006.15.566.
2
Albumin marks pseudopodia of astrocytoma cells responding to hepatocyte growth factor or serum.白蛋白标记对肝细胞生长因子或血清作出反应的星形细胞瘤细胞的伪足。
Lab Invest. 2006 Nov;86(11):1103-14. doi: 10.1038/labinvest.3700470. Epub 2006 Sep 11.
3
Multipotent, dedifferentiated cancer stem-like cells from brain gliomas.来自脑胶质瘤的多能、去分化的癌症干细胞样细胞。
Stem Cells Dev. 2006 Jun;15(3):423-35. doi: 10.1089/scd.2006.15.423.
4
Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation.鉴定调节神经干/祖细胞分化的星形胶质细胞表达因子。
Stem Cells Dev. 2006 Jun;15(3):407-21. doi: 10.1089/scd.2006.15.407.
5
Redefining cellular phenotypy based on embryonic, adult, and cancer stem cell biology.基于胚胎、成体和癌症干细胞生物学重新定义细胞表型。
Brain Pathol. 2006 Apr;16(2):169-80. doi: 10.1111/j.1750-3639.2006.00011.x.
6
Neural subtype specification from embryonic stem cells.从胚胎干细胞中进行神经亚型的特异性分化。
Brain Pathol. 2006 Apr;16(2):132-42. doi: 10.1111/j.1750-3639.2006.00008.x.
7
Brain tumour stem cells.脑肿瘤干细胞
Nat Rev Cancer. 2006 Jun;6(6):425-36. doi: 10.1038/nrc1889.
8
Brain tumor stem cells: new targets for clinical treatments?脑肿瘤干细胞：临床治疗的新靶点？
Neurosurg Focus. 2006 Apr 15;20(4):E27. doi: 10.3171/foc.2006.20.4.17.
9
Conserved roles for Oct4 homologues in maintaining multipotency during early vertebrate development.Oct4同源物在脊椎动物早期发育过程中维持多能性方面的保守作用。
Development. 2006 May;133(10):2011-22. doi: 10.1242/dev.02362.
10
Brain cancer stem-like cells.脑癌干细胞样细胞
Eur J Cancer. 2006 Jun;42(9):1237-42. doi: 10.1016/j.ejca.2006.01.038. Epub 2006 May 2.
Zotero 插件