[既往接种和未接种疫苗患者麻疹病毒感染的实验室诊断特点]

[Peculiarity of the laboratory diagnostic of the measles virus infection in previously vaccinated and unvaccinated patients].

作者信息

Mamaeva T A, Lipskaia G Iu, Naumova M A, Shul'ga S V, Mulders M, Featherstone D A, Zav'ialova L A, Chernysheva E V, Zamiatina E P, Kuznetsova N N

出版信息

Vopr Virusol. 2012 Sep-Oct;57(5):21-6.

PMID:23248855

Abstract

119 specimens of blood sera collected from measles cases with different vaccination history (aged 4 months to 48 years) on 5th-6th days after rash onset were Investigated using EIA. The obtained results showed that the primary immune response (PIR) was developed in 59 patients; the secondary immune response (SIR) was developed in 60 patients with a significant increase in the specific high avidity IgG (22.34 IU/ml +/- 3.2). The specific IgM were detected in 100% cases studied with capture ELISA in both previously vaccinated and unvaccinated individuals of different age. The specific IgM were detected by indirect ELISA in 100% cases in unvaccinated patients, while IgM positive sera was defined only in 23.3% of individuals with SIR. It was concluded that measles virus infection in previously vaccinated and unvaccinated adults had clinical differences. The role of patients with SIR in virus transmission was discussed.

摘要

对119份在出疹后第5至6天采集的、来自不同疫苗接种史（年龄4个月至48岁）麻疹病例的血清样本，采用酶免疫分析法（EIA）进行研究。结果显示，59例患者出现了初次免疫应答（PIR）；60例患者出现了二次免疫应答（SIR），特异性高亲和力IgG显著升高（22.34 IU/ml ± 3.2）。在不同年龄的既往接种和未接种个体中，用捕获ELISA法在100%的研究病例中检测到特异性IgM。用间接ELISA法在100%未接种疫苗的患者病例中检测到特异性IgM，而在二次免疫应答患者中仅23.3%的个体血清IgM呈阳性。得出结论：既往接种和未接种疫苗的成年人感染麻疹病毒存在临床差异。并讨论了二次免疫应答患者在病毒传播中的作用。