Mamaeva T A, Naumova M A, Zheleznova N V, Lipskaia G Iu, Mulders M, Featherstone D A
Vopr Virusol. 2013 Sep-Oct;58(5):43-8.
Nine commercial kits of "captured" and "indirect" format ELISA assay for the detection of specific IgM and IgG in sera of patients with measles were compared to each other. 72 sera specimens from typical medium-severity cases from a measles outbreak (2010) were collected on the 5-6th day after the rash onset. IgM was detected with "capture" tests (Vecto-Measles IgM, Vector Best, Measles IgM capture EIA, Microimmune Ltd) close to 100% of cases, irrespectively to the age and the initial vaccination status of the patients. The IgM result was negative in 23.6% by average while investigating using "indirect" format tests (Enzygnost Anti-Measles Virusll/IgM, Siemens; Anti-Measles Viruses ELISA (IgM), Eurominimum, Virion-Serion IgM (GmbH). These cases were in adults, the majority of which had 1-2 vaccinations in the past. The analysis of the presented data shows high correlation connection between the tests used and high confidence level for OD IgM and IgG of the sera of the patients with the primary and secondary immune response.
对九种用于检测麻疹患者血清中特异性 IgM 和 IgG 的“捕获”和“间接”格式 ELISA 检测的商业试剂盒进行了相互比较。从 2010 年麻疹暴发的典型中重度病例中收集了 72 份血清标本，于出疹后第 5 - 6 天采集。通过“捕获”试验（Vecto-Measles IgM、Vector Best、Measles IgM capture EIA、Microimmune Ltd）检测 IgM，无论患者年龄和初始疫苗接种状况如何，接近 100%的病例均可检测到。而使用“间接”格式试验（Enzygnost Anti-Measles Virusll/IgM，西门子；Anti-Measles Viruses ELISA (IgM)，Eurominimum；Virion-Serion IgM (GmbH)）进行检测时，IgM 结果平均有 23.6%为阴性。这些病例均为成年人，其中大多数过去接种过 1 - 2 次疫苗。对所呈现数据的分析表明，所使用的检测方法之间具有高度相关性，并且对具有初次和二次免疫反应的患者血清的 OD IgM 和 IgG 具有较高的置信水平。
