al-Ahdal M N, al-Hussain K, Thorogood R J, Reilly H C, Wilson J D
Department of Biological and Medical Research, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
J Trop Med Hyg. 1990 Apr;93(2):98-105.
The protein constituents of saliva, salivary gland extracts, haemolymph and whole body extract from the Culex molestus mosquito were compared by polyacrylamide gel electrophoresis. When developed by the sensitive silver staining technique, saliva and salivary gland extract were found to contain 15 comparable protein bands. Salivary gland extract contained additional bands, presumed to be structural proteins, and saliva contained some unique protein bands which were not present in the gland extract, possibly originating in the salivary stylet lining. Some differences were found in the protein components of salivary gland extracts prepared from mosquitoes of different ages. Salivary proteins were found to be poorly represented in both haemolymph and whole body extracts. Immunoblotting of salivary gland extract with antibody raised against pure saliva exhibited binding to at least nine protein bands indicating the potential for using salivary gland extracts in place of mosquito saliva for further studies.
通过聚丙烯酰胺凝胶电泳对致倦库蚊唾液、唾液腺提取物、血淋巴和全身提取物中的蛋白质成分进行了比较。采用灵敏的银染技术显色时，发现唾液和唾液腺提取物含有15条可比的蛋白带。唾液腺提取物含有额外的条带，推测为结构蛋白，而唾液含有一些腺提取物中不存在的独特蛋白带，可能起源于唾液针管内衬。在不同年龄蚊子制备的唾液腺提取物的蛋白质成分中发现了一些差异。唾液蛋白在血淋巴和全身提取物中的含量均较低。用针对纯唾液产生的抗体对唾液腺提取物进行免疫印迹，结果显示与至少九条蛋白带结合，这表明在进一步研究中可用唾液腺提取物替代蚊子唾液。
