Peng Z, Simons F E
Department of Pediatrics and Child Health, University of Manitoba, Canada.
Ann Allergy Asthma Immunol. 1996 Nov;77(5):371-6. doi: 10.1016/S1081-1206(10)63335-2.
Commercial extracts are available for the diagnosis and treatment of mosquito allergy, but their antigen content has never been analyzed.
We wanted to analyze commercially available mosquito extracts and to compare these extracts with different laboratory preparations.
Seven commercially available mosquito whole body extracts from six companies and four laboratory mosquito preparations including saliva extract were studied. Epicutaneous tests and measurement of protein concentration were performed. Protein components were identified by sodium sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and silver stain. IgE and IgG binding antigens were analyzed by SDS-PAGE and immunoblotting with sera from mosquito-allergic subjects.
The seven commercial materials produced wheals and papules ranging from 0 to 36 mm2. Their protein concentrations varied from 0.1 to 4.9 mg/mL. There were significant differences in their protein and antigen components. Some extracts contained multiple highly immunoreactive proteins and IgE- and IgG-binding antigens that are not present in mosquito saliva, but few actual salivary antigens. In the four laboratory preparations, rank ordered from whole body, head and thorax, salivary gland to saliva extracts, the amount of salivary antigens significantly increased, while non-salivary proteins and antigens significantly decreased.
Commercial mosquito extracts should be standardized. Purer mosquito extracts should be used in diagnosis and immunotherapy of mosquito allergy.
有用于蚊虫过敏诊断和治疗的商业提取物，但从未对其抗原含量进行过分析。
我们想分析市售的蚊虫提取物，并将这些提取物与不同的实验室制备物进行比较。
研究了来自6家公司的7种市售蚊虫全身体提取物以及4种实验室蚊虫制备物，包括唾液提取物。进行了皮肤点刺试验和蛋白质浓度测定。通过硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和银染鉴定蛋白质成分。通过SDS-PAGE和用蚊虫过敏受试者的血清进行免疫印迹分析IgE和IgG结合抗原。
这7种商业材料产生的风团和丘疹面积为0至36平方毫米。它们的蛋白质浓度在0.1至4.9毫克/毫升之间变化。它们的蛋白质和抗原成分存在显著差异。一些提取物含有多种高度免疫反应性蛋白质以及蚊虫唾液中不存在的IgE和IgG结合抗原，但实际的唾液抗原很少。在4种实验室制备物中，从全身体、头胸部、唾液腺到唾液提取物依次排序，唾液抗原的量显著增加，而非唾液蛋白质和抗原显著减少。
商业蚊虫提取物应标准化。应使用更纯的蚊虫提取物进行蚊虫过敏的诊断和免疫治疗。
