Institute of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech Republic.
Plant Physiol Biochem. 2013 Feb;63:82-8. doi: 10.1016/j.plaphy.2012.11.019. Epub 2012 Nov 29.
Accumulation of secondary metabolites (general phenols, naphthodianthrones and phloroglucinol hyperforin) in Hypericum perforatum and Hypericum canariense after application of the inhibitor (2-aminoindane-2-phosphonic acid, AIP) of the pivotal enzyme of general phenylpropanoid pathway (phenylalanine ammonia-lyase, PAL) was studied. Shoots of H. perforatum revealed more expressive growth depression, concomitantly with the inhibition of PAL activity (-60%) and decrease in soluble phenols and individual phenolic acids in response to AIP. Hypericins (hypericin, pseudohypericin and protohypericin) decreased while hyperforin increased in AIP-cultured H. perforatum. On the contrary, growth changes, decreases in soluble phenols and individual phenolic acids were less-visible in H. canariense. This was also reflected in restoration of PAL activity (+330%) and selected flavonoids even increased. Hypericins and hyperforin were present in several orders of magnitude lower amounts in comparison with H. perforatum. Increase in proline indicates potential compensatory antioxidative mechanism if phenols are depleted. Microscopy revealed also differences in secondary xylem formation and lignification between species after exposure to AIP.
研究了应用苯丙氨酸解氨酶（PAL）关键酶抑制剂（2-氨基吲哚-2-磷酸，AIP）后贯叶金丝桃和金丝桃中次生代谢产物（总酚类、萘并二蒽酮和间苯三酚金丝桃素）的积累情况。H. perforatum 的芽显示出更明显的生长抑制，同时 PAL 活性受到抑制（-60%），可溶性酚类和个别酚酸减少。AIP 培养的 H. perforatum 中的金丝桃素（金丝桃素、伪金丝桃素和原金丝桃素）减少，而金丝桃素增加。相反，H. canariense 中的生长变化、可溶性酚类和个别酚酸减少不太明显。这也反映在 PAL 活性的恢复（+330%）和某些类黄酮的增加。与 H. perforatum 相比，H. canariense 中的金丝桃素和金丝桃素含量低几个数量级。脯氨酸的增加表明，如果酚类物质被耗尽，可能会产生潜在的补偿抗氧化机制。显微镜还揭示了 AIP 暴露后两种物种次生木质部形成和木质素化的差异。
