Rao G, Xia E, Richardson A
Department of Biological Sciences, Illinois State University, Normal 61716.
Mech Ageing Dev. 1990 Mar 31;53(1):49-60. doi: 10.1016/0047-6374(90)90033-c.
Age-related changes in the activities of superoxide dismutase, catalase, and glutathione peroxidase were determined in brain, heart, hepatocytes, intestinal mucosa, and kidney from male Fischer F344 rats. Superoxide dismutase activity decreased significantly with age in all five tissues studied. The activity of catalase decreased with age in brain, hepatocytes, and kidney while glutathione peroxidase activity decreased significantly with age only in intestinal mucosa and kidney. The relative levels of superoxide dismutase, catalase, and glutathione peroxidase mRNA were measured in brain, hepatocytes, and kidney. An age-related decrease in SOD and catalase mRNA was observed for brain, hepatocytes, and kidney. GPX mRNA levels decreased with age in hepatocytes and kidney but did not change with age in brain. In general, the age-related changes in the activities of SOD, catalase, and GPX were paralleled by a similar change in the relative level of the mRNAs coding for these enzymes.
测定了雄性Fischer F344大鼠脑、心脏、肝细胞、肠黏膜和肾脏中与年龄相关的超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性变化。在所研究的所有五种组织中，超氧化物歧化酶活性均随年龄显著降低。过氧化氢酶活性在脑、肝细胞和肾脏中随年龄降低，而谷胱甘肽过氧化物酶活性仅在肠黏膜和肾脏中随年龄显著降低。测定了脑、肝细胞和肾脏中超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶mRNA的相对水平。在脑、肝细胞和肾脏中观察到超氧化物歧化酶和过氧化氢酶mRNA随年龄降低。谷胱甘肽过氧化物酶mRNA水平在肝细胞和肾脏中随年龄降低，但在脑中不随年龄变化。一般来说，超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性随年龄的变化与编码这些酶的mRNA相对水平的类似变化平行。
