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Phosphorus 31 NMR of neuroblastoma clonal lines. Effect of cell confluency state and dibutyryl cyclic AMP.

作者信息

Pettegrew J W, Glonek T, Baskin F, Rosenberg R N

出版信息

Neurochem Res. 1979 Dec;4(6):795-802. doi: 10.1007/BF00964475.

DOI:10.1007/BF00964475
PMID:232546
Abstract

Phosphorus nuclear magnetic resonance is used to study changes in the levels of the major phosphate-containing intermediary metabolites concomitant with induced cellular differentiation in the N-18 and C-46 neuroblastoma clonal lines. The study reveals differences between the 31P-NMR profiles of the two clonal lines and also striking differences attendant to dibutyryl cAMP-mediated morphological differentiation in the N-18 clone. Phosphorus-31 NMR would appear to provide a new technique with which to study genetic differentiation.

摘要

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本文引用的文献

1
Proteins from morphologically differentiated neuroblastoma cells promote tubulin polymerization.来自形态分化的神经母细胞瘤细胞的蛋白质促进微管蛋白聚合。
J Cell Biol. 1978 Feb;76(2):547-55. doi: 10.1083/jcb.76.2.547.
2
Regulation of axon formation by clonal lines of a neural tumor.神经肿瘤克隆系对轴突形成的调控。
Proc Natl Acad Sci U S A. 1970 May;66(1):160-7. doi: 10.1073/pnas.66.1.160.
3
Observation of tissue metabolites using 31P nuclear magnetic resonance.使用31P核磁共振观察组织代谢物
Nature. 1974 Nov 22;252(5481):285-7. doi: 10.1038/252285a0.
4
Analysis of living tissue by phosphorus-31 magnetic resonance.通过磷-31磁共振对活体组织进行分析。
Science. 1977 Jan 14;195(4274):145-9. doi: 10.1126/science.188132.
5
Experimental chemotherapy of neuroblastoma. II. Increased thymidylate synthetase activity in a 5-fluorodeoxyuridine-resistant variant of mouse neuroblastoma.
Mol Pharmacol. 1975 Jan;11(1):105-17.
6
Analysis of phosphate metabolites, the intracellular pH, and the state of adenosine triphosphate in intact muscle by phosphorus nuclear magnetic resonance.利用磷核磁共振分析完整肌肉中的磷酸盐代谢物、细胞内pH值和三磷酸腺苷状态。
J Biol Chem. 1976 May 10;251(9):2584-91.