基于氧化石墨探针的人血清白蛋白新型简单共振瑞利散射法
A new and simple resonance Rayleigh scattering method for human serum albumin using graphite oxide as probe.
机构信息
State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Science and Technology of China, Guilin, China; Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education of China, Guilin, China; Guangxi Normal University, Guilin, China.
出版信息
Luminescence. 2013 Nov-Dec;28(6):842-6. doi: 10.1002/bio.2443. Epub 2012 Dec 17.
Graphite oxide (GO) was prepared by the Hummer procedure, and can be dispersed to stable colloid solution by ultrasonic wave. The GO exhibited an absorption peak at 313 nm, and a resonance Rayleigh scattering (RRS) peak at 490 nm. In pH 4.6 HAc-NaAc buffer solution, human serum albumin (HSA) combined with GO probe to form large HSA-GO particles that caused the RRS peak increasing at 490 nm. The increased RRS intensity was linear to HSA concentration in the range 0.50-200 µg/mL. Thus, a new and simple RRS method was proposed for the determination of HSA in samples, with a recovery of 98.1-104%.
氧化石墨（GO）通过 Hummer 法制备，经超声波分散可得到稳定的胶体溶液。GO 在 313nm 处有吸收峰，在 490nm 处有共振瑞利散射（RRS）峰。在 pH4.6 的 HAc-NaAc 缓冲溶液中，人血清白蛋白（HSA）与 GO 探针结合形成大的 HSA-GO 颗粒，导致在 490nm 处的 RRS 峰增强。在 0.50-200μg/mL 范围内，RRS 强度的增加与 HSA 的浓度呈线性关系。因此，提出了一种新的、简单的 RRS 法用于样品中 HSA 的测定，回收率为 98.1-104%。
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