Bozchaloei Shabnam Soltani, Gong Siew-Ging, Dehpour Ahmad R, Farrokh Parisa, Khoshayand Mohammad R, Oskoui Mahvash
Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran.
J Investig Clin Dent. 2013 Nov;4(4):233-9. doi: 10.1111/jicd.12020. Epub 2012 Dec 17.
Caffeine is one of the most widely consumed behaviorally active substances in the world. Although its effects on the central nervous system and bone metabolism have been documented, as yet there is no report on its effect on tissues in the oral cavity. In this study we analyzed the viability of human gingival fibroblasts (HGF) and alkaline phosphatase (ALP) enzyme activity after exposure to different concentrations of caffeine for different exposure time periods.
The HGF were cultured with different concentrations of caffeine. Viability of cells exposed to caffeine was analyzed by the 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay to assess mitochondrial dehydrogenase activity. The activity of ALP was analyzed at specific time intervals after caffeine addition.
Our results showed that caffeine of concentrations <1 mm did not affect the viability of HGF and the ALP enzyme activity. Nevertheless, caffeine at 5 and 10 mm dramatically decreased the viability and ALP activity of the cells after 4 days such that, by day 9, the viability of cells declined to near zero in the 10 mm group.
These results provided evidence that caffeine in high concentrations can decrease cellular viability and ALP activity in HGF.
咖啡因是世界上消费最广泛的具有行为活性的物质之一。尽管其对中枢神经系统和骨代谢的影响已有文献记载，但尚无关于其对口腔组织影响的报道。在本研究中，我们分析了人牙龈成纤维细胞（HGF）在暴露于不同浓度咖啡因不同时间段后的活力以及碱性磷酸酶（ALP）的活性。
将HGF与不同浓度的咖啡因一起培养。通过3-（4,5-二甲基噻唑-2）-2,5-二苯基四氮唑溴盐（MTT）法分析暴露于咖啡因的细胞活力，以评估线粒体脱氢酶活性。在添加咖啡因后的特定时间间隔分析ALP的活性。
我们的结果表明，浓度<1 mM的咖啡因不会影响HGF的活力和ALP活性。然而，5 mM和10 mM的咖啡因在4天后显著降低了细胞的活力和ALP活性，以至于到第9天，10 mM组细胞的活力降至接近零。
这些结果证明高浓度咖啡因可降低HGF的细胞活力和ALP活性。
