Abe T, Hara Y, Abe Y, Aida Y, Maeda K
Department of Periodontology and Endodontology, Faculty of Dentistry, Kyushu University, Fukuoka, Japan.
J Dent Res. 1998 Sep;77(9):1700-7. doi: 10.1177/00220345980770090601.
We have previously reported that the increased expression of alkaline phosphatase (ALP) activity is a phenotypic characteristic of gingival fibroblasts present in chronic inflammatory periodontal lesions. We hypothesized that ALP might be induced in gingival fibroblasts by environmental factors. In the present study, we investigated the factors influencing the induction of ALP expression in fibroblasts derived from healthy human gingiva. The withdrawal of serum from confluent cultures of fibroblasts increased the number of cells positive for ALP activity and protein, without their proliferation. Suramin, a growth factor antagonist, induced ALP expression in cells cultured with serum. Serum re-addition or exposure to platelet-derived growth factor-AB and/or insulin-like growth factor I suppressed ALP induction and caused cell growth. ALP-positive cells could survive for up to 6 weeks after serum deprivation, a condition inducing cell death via apoptosis. These results demonstrate that serum or growth factor deprivation induces the expression of ALP in gingival fibroblasts. ALP expression is negatively correlated with cell growth and accompanied by a change into serum-growth-factor-independent survival.
我们之前曾报道，碱性磷酸酶（ALP）活性表达增加是慢性炎症性牙周病变中牙龈成纤维细胞的一种表型特征。我们推测ALP可能是由环境因素在牙龈成纤维细胞中诱导产生的。在本研究中，我们调查了影响健康人牙龈来源的成纤维细胞中ALP表达诱导的因素。从汇合的成纤维细胞培养物中去除血清，增加了ALP活性和蛋白阳性细胞的数量，且细胞未增殖。苏拉明，一种生长因子拮抗剂，在有血清培养的细胞中诱导ALP表达。重新添加血清或暴露于血小板衍生生长因子-AB和/或胰岛素样生长因子I可抑制ALP诱导并促进细胞生长。血清剥夺后，ALP阳性细胞可存活长达6周，血清剥夺是一种通过凋亡诱导细胞死亡的条件。这些结果表明，血清或生长因子剥夺可诱导牙龈成纤维细胞中ALP的表达。ALP表达与细胞生长呈负相关，并伴随着向血清生长因子非依赖性存活的转变。
