Postgrado en Fitosanidad-Entomología y Acarología, Colegio de Postgraduados, Carretera Mexico-Texcoco, Montecillo, Estado de Mexico, Mexico.
Pest Manag Sci. 2013 Jul;69(7):874-82. doi: 10.1002/ps.3452. Epub 2012 Dec 18.
Maconellicoccus hirsutus Green is a widely distributed pest of numerous crops. Although synthetic pesticides are used to control this pest, entomopathogenic fungi may provide an alternative control mechanism. Three experiments were carried out to select a fungal isolate with the potential to be used as a microbial control agent. The in vitro growth of five isolates of Beauveria bassiana sensu lato (Bals.) Vuill and Metarhizium anisopliae sensu lato (Metschn.) Sorokín, along with three isolates of Lecanicillium lecanii (Zimm.) Zare & W. Gams and Isaria fumosoroseus (Wize), was assessed at four temperatures. The in vivo sporulation of eight selected isolates was then evaluated, followed by the susceptibility of third-instar M. hirsutus to a single dose (1 × 10(8) conidia mL(-1) ) of each of these isolates.
Growth was greatest by isolates of I. fumosoroseus and L. lecanii at 15 and 20 °C and by isolates of M. anisopliae at 25 and 30 °C. In vivo conidium production was greatest when infected with B. bassiana isolate GHA and M. anisopliae isolate Ma65. Mortality was greatest when inoculated with M. anisopliae isolates Ma65 and Ma129.
Isolate Ma65 shows the best potential to be developed as a microbial control agent for M. hirsutus.
多毛实盘孢菌（Maconellicoccus hirsutus Green）是一种广泛分布的多种作物害虫。虽然合成农药被用于控制这种害虫，但昆虫病原真菌可能提供一种替代的控制机制。进行了三项实验来选择一种具有作为微生物防治剂潜力的真菌分离物。在四个温度下评估了五种贝氏拟青霉（Beauveria bassiana sensu lato（Bals.）Vuill）和金龟子绿僵菌（Metarhizium anisopliae sensu lato（Metschn.）Sorokín）分离物以及三种蜡蚧轮枝菌（Lecanicillium lecanii（Zimm.）Zare & W. Gams）和玫烟色棒束孢（Isaria fumosoroseus（Wize））的体外生长情况。然后评估了八个选定分离物的体内孢子形成情况，随后评估了第三龄多毛实盘孢对这些分离物中每一种的单一剂量（1×10(8)个分生孢子 mL(-1)）的敏感性。
在 15 和 20°C 时，玫烟色棒束孢和蜡蚧轮枝菌的分离物的生长最大，在 25 和 30°C 时，金龟子绿僵菌的分离物的生长最大。当感染贝氏拟青霉分离物 GHA 和金龟子绿僵菌分离物 Ma65 时，体内分生孢子产生最大。接种金龟子绿僵菌分离物 Ma65 和 Ma129 时死亡率最高。
分离物 Ma65 显示出作为多毛实盘孢微生物防治剂的最大潜力。
