在粟酒裂殖酵母中，受花椰菜花叶病毒35s启动子控制的β-葡萄糖醛酸酶基因的表达。
Expression of the beta-glucuronidase gene under the control of the CaMV 35s promoter in Schizosaccharomyces pombe.
作者信息
Pobjecky N, Rosenberg G H, Dinter-Gottlieb G, Käufer N F
机构信息
Department of Bioscience and Biotechnology, Drexel University, Philadelphia, PA 19104.
出版信息
Mol Gen Genet. 1990 Jan;220(2):314-6. doi: 10.1007/BF00260500.
PMID:2325625
Abstract
We have transformed Schizosaccharomyces pombe with the beta-glucuronidase (GUS) gene from Escherichia coli under the control of the plant cauliflower mosaic virus (CaMV) 35S promoter element. Efficient expression of GUS enzyme was observed. Moreover, transcription initiated at a unique site identical to that used in plant cells.
摘要
我们已用来自大肠杆菌的β-葡萄糖醛酸酶（GUS）基因转化粟酒裂殖酵母，该基因受植物花椰菜花叶病毒（CaMV）35S启动子元件调控。观察到GUS酶的高效表达。此外，转录起始于与植物细胞中相同的独特位点。
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