鉴定控制软骨基质蛋白基因表达的正负调控区域。
Identification of positive and negative regulatory regions controlling expression of the cartilage matrix protein gene.
作者信息
Kiss I, Bösze Z, Szabó P, Altanchimeg R, Barta E, Deák F
机构信息
Institute of Biochemistry, Hungarian Academy of Sciences, Szeged.
出版信息
Mol Cell Biol. 1990 May;10(5):2432-6. doi: 10.1128/mcb.10.5.2432-2436.1990.
Abstract
A complex pattern of regulation of the cartilage matrix protein gene was revealed by transient expression experiments. A minimal promoter from positions -15 to +64 functioned in chondrocytes and fibroblasts. An enhancer located in the first intron exerted chondrocyte-specific stimulation on the minimal promoter activity. The same fragment, however, had a negative effect in fibroblasts. Between -334 and -15, a silencer was found which inhibited the gene expression driven from its homologous as well as heterologous promoters both in chondrocytes and fibroblasts. Additional positive and negative control regions were mapped further upstream of the promoter.
摘要
瞬时表达实验揭示了软骨基质蛋白基因的复杂调控模式。从 -15 到 +64 位的最小启动子在软骨细胞和成纤维细胞中发挥作用。位于第一个内含子中的增强子对最小启动子活性发挥软骨细胞特异性刺激作用。然而，相同的片段在成纤维细胞中具有负面影响。在 -334 和 -15 之间，发现了一个沉默子，它在软骨细胞和成纤维细胞中均抑制由其同源以及异源启动子驱动的基因表达。在启动子上游更远的位置定位了其他正负调控区域。
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