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引用本文的文献

1
Highly conserved proximal promoter element harbouring paired Sox9-binding sites contributes to the tissue- and developmental stage-specific activity of the matrilin-1 gene.含有配对Sox9结合位点的高度保守的近端启动子元件有助于母连蛋白-1基因的组织和发育阶段特异性活性。
Biochem J. 2005 Aug 1;389(Pt 3):705-16. doi: 10.1042/BJ20050214.
2
Negative regulation of transcription in eukaryotes.真核生物转录的负调控
Biochem J. 1993 Dec 15;296 ( Pt 3)(Pt 3):521-41. doi: 10.1042/bj2960521.
3
Endothelial-cell-specific regulation of von Willebrand factor gene expression.血管性血友病因子基因表达的内皮细胞特异性调控。
Mol Cell Biol. 1994 Feb;14(2):999-1008. doi: 10.1128/mcb.14.2.999-1008.1994.
4
Multiple positive and negative elements regulate human brain creatine kinase gene expression.多种正负调控元件调节人脑中肌酸激酶基因的表达。
Nucleic Acids Res. 1991 Nov 25;19(22):6231-40. doi: 10.1093/nar/19.22.6231.

本文引用的文献

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Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.在哺乳动物细胞中表达氯霉素乙酰转移酶的重组基因组。
Mol Cell Biol. 1982 Sep;2(9):1044-51. doi: 10.1128/mcb.2.9.1044-1051.1982.
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Location and function of retroviral and SV40 sequences that enhance biochemical transformation after microinjection of DNA.逆转录病毒和SV40序列在DNA显微注射后增强生化转化的位置及功能。
Cell. 1983 Jul;33(3):705-16. doi: 10.1016/0092-8674(83)90013-2.
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Enhancer elements.增强子元件
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The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection.劳氏肉瘤病毒长末端重复序列通过DNA介导转染导入多种真核细胞时是一个强启动子。
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Examination of glucocorticoid sensitivity and receptor content of hepatoma cell lines.肝癌细胞系糖皮质激素敏感性及受体含量检测
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A new technique for the assay of infectivity of human adenovirus 5 DNA.一种检测人腺病毒5型DNA感染性的新技术。
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Characterization of differentiated and dedifferentiated clones from a rat hepatoma.大鼠肝癌分化和去分化克隆的特征分析
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8
The human beta-interferon gene enhancer is under negative control.人类β-干扰素基因增强子处于负调控之下。
Cell. 1986 May 23;45(4):601-10. doi: 10.1016/0092-8674(86)90292-8.
9
Identification of a phenotype-specific enhancer in the first intron of the rat collagen II gene.大鼠胶原蛋白II基因第一个内含子中一种表型特异性增强子的鉴定。
Proc Natl Acad Sci U S A. 1987 Dec;84(24):8864-8. doi: 10.1073/pnas.84.24.8864.
10
Structure of the chicken link protein gene: exons correlate with the protein domains.鸡连接蛋白基因的结构：外显子与蛋白质结构域相关。
Proc Natl Acad Sci U S A. 1987 Sep;84(18):6399-403. doi: 10.1073/pnas.84.18.6399.

鉴定控制软骨基质蛋白基因表达的正负调控区域。

Identification of positive and negative regulatory regions controlling expression of the cartilage matrix protein gene.

作者信息

Kiss I, Bösze Z, Szabó P, Altanchimeg R, Barta E, Deák F

机构信息

Institute of Biochemistry, Hungarian Academy of Sciences, Szeged.

出版信息

Mol Cell Biol. 1990 May;10(5):2432-6. doi: 10.1128/mcb.10.5.2432-2436.1990.

DOI:10.1128/mcb.10.5.2432-2436.1990
PMID:2325660
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC360594/
Abstract

A complex pattern of regulation of the cartilage matrix protein gene was revealed by transient expression experiments. A minimal promoter from positions -15 to +64 functioned in chondrocytes and fibroblasts. An enhancer located in the first intron exerted chondrocyte-specific stimulation on the minimal promoter activity. The same fragment, however, had a negative effect in fibroblasts. Between -334 and -15, a silencer was found which inhibited the gene expression driven from its homologous as well as heterologous promoters both in chondrocytes and fibroblasts. Additional positive and negative control regions were mapped further upstream of the promoter.

摘要

瞬时表达实验揭示了软骨基质蛋白基因的复杂调控模式。从 -15 到 +64 位的最小启动子在软骨细胞和成纤维细胞中发挥作用。位于第一个内含子中的增强子对最小启动子活性发挥软骨细胞特异性刺激作用。然而，相同的片段在成纤维细胞中具有负面影响。在 -334 和 -15 之间，发现了一个沉默子，它在软骨细胞和成纤维细胞中均抑制由其同源以及异源启动子驱动的基因表达。在启动子上游更远的位置定位了其他正负调控区域。