Chen Jia-yu, Yu Wei, Liu Wei-wei, Li Bo, Li Yang-qiu, Yang Li-jian, Chen Shao-hua
Guangzhou 12th Municipal People's Hospital, Guangzhou 510620, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2012 Oct;30(10):739-41.
To observe the T cell subsets and blood cells in the peripheral blood of workers exposed to low levels of benzene for one year, and to investigate the relationship between T cell function impairment and benzene-induced hematopoietic injury after benzene exposure.
Eighty-eight workers (58 males and 30 females, aged 18 ∼ 22 years) who just began to work in the workshop of a paint factory with exposure to benzene in Guangzhou, China were assigned to experimental group, and 88 workers (58 males and 30 females, aged 18 ∼ 25 years) who worked in the workshop without exposure to benzene were selected as controls. The blood samples of the workers were examined once every 4 months to measure the percentages of peripheral T cell subsets and peripheral blood cell counts in the one-year study. The benzene concentrations at operation points were also measured.
The peripheral blood cell counts in the benzene-exposed workers had no significant changes in the first and second examinations; the white blood cell (WBC) counts in the experimental group in the third and fourth examinations were significantly lower than that in the control group [(6.4 ± 3.0)×10(9)/L and (6.3 ± 2.7)×10(9)/L vs (7.3 ± 3.0)×10(9)/L, P < 0.05], and the platelet (PLT) count in the experimental group in the fourth examination was also significantly lower than that in the control group[(179 ± 74)×10(9)/L vs (189 ± 70)×10(9)/L, P < 0.05]. Compared with those in the control group (CD4+: 54.29 ± 12.78%, CD8+: 37.25 ± 12.30%), the percentage of CD3+ T cells in the experimental group increased in the third examination; the percentage of CD4+ T cells in the experimental group decreased continuously in the second, third, and fourth examinations (50.77 ± 11.05%, 45.40 ± 9.41%, and 41.27 ± 10.62%), while the percentage of CD8+ T cells in the experimental group kept increasing (46.07 ± 10.18%, 50.36 ± 10.62%, and 56.40 ± 9.41%) (P < 0.05).
The change in T cell subsets precedes that in the blood system in the workers exposed to low levels of benzene.
观察接触低浓度苯1年的工人外周血T细胞亚群及血细胞情况，探讨苯接触后T细胞功能损害与苯所致造血损伤之间的关系。
选取中国广州某油漆厂车间刚开始接触苯的88名工人（男58名，女30名，年龄18～22岁）作为实验组，选取在无苯接触车间工作的88名工人（男58名，女30名，年龄18～25岁）作为对照组。在为期1年的研究中，每隔4个月对工人的血样进行检测，以测定外周血T细胞亚群百分比和外周血细胞计数。同时测量操作点的苯浓度。
苯接触工人外周血细胞计数在第1次和第2次检测时无明显变化；实验组第3次和第4次检测时白细胞（WBC）计数显著低于对照组[（6.4±3.0）×10⁹/L和（6.3±2.7）×10⁹/L vs（7.3±3.0）×10⁹/L，P<0.05]，实验组第4次检测时血小板（PLT）计数也显著低于对照组[（179±74）×10⁹/L vs（189±70）×10⁹/L，P<0.05]。与对照组（CD4⁺：54.29±12.78%，CD8⁺：37.25±12.30%）相比，实验组第3次检测时CD3⁺T细胞百分比升高；实验组第2次、第3次和第4次检测时CD4⁺T细胞百分比持续下降（50.77±11.05%、45.40±9.41%和41.27±10.62%），而实验组CD8⁺T细胞百分比持续升高（46.07±10.18%、50.36±10.62%和56.40±9.41%）（P<0.05）。
低浓度苯接触工人T细胞亚群变化先于血液系统变化。
